PathoScreen® Kit for Phytophthora (Phyt)

The Phytophthora (Phyt) ELISA test system is intended for use with plant samples exhibiting symptoms of Phytophthora infection. This test can also be used to test fungal culture samples. The Phytophthora ELISA detects Phytophthora to the genus level and cannot differentiate species. For a full list of species that have experimentally detected, click here.

This test is the approved high throughput screening method in a U.S. national survey for Phytophthora ramorum, the causal agent of sudden oak death disease.

If you would prefer to add a field-deployable assay to your testing toolkit, please check out Agdia's Phyt ImmunoStrips®

Click here for other product documents such as the User Guide or Buffer Instructions.

Included:

  • Antibody-coated 96-well microtiter plates
  • Alkaline phosphatase enzyme conjugate
  • RUB6, 1X Ready to use
  • PNP substrate tablets, 5 mg each
  • PNP substrate buffer, 5X concentrate
  • Positive control
  • PBST buffer, 20X concentrate, 50 mL
  • General extract buffer 2, GEB2 powder
  • User Guide

Required but Not Included:

For a full list of included items, including sizes, please see the Content List.

Validation Report Summary

Test Label: Alkaline Phosphatase
Test Format: Double Antibody Sandwich (DAS) ELISA
Capture Reagent: Polyclonal
Detection Reagent: Monoclonal

Full Validation Report


Analytical Sensitivity
Limit of detection: 1:1,280 dilution of infected tissue (pathogen titer unknown)

Phytophthora Species Detected1
Phytophthora alticola-type Phytophthora asparagi
Phytophthora alni Phytophthora boehmeriae
Phytophthora bisheria Phytophthora cactorum
Phytophthora cajani Phytophthora cambivora
Phytophthora canlensis Phytophthora capsici
Phytophthora cinnamomi var parvispora Phytophthora cinnamomi var robiniae
Phytophthora citricola Phytophthora citrophthora
Phytophthora cryptogea Phytophthora drechsleri
Phytophthora europaea Phytophthora erythroseptica
Phytophthora fragariae var fragariae Phytophthora fragariae var rubi
Phytophthora glovera Phytophthora gonapodyides
Phytophthora heveae Phytophthora hibernalis
Phytophthora infestans Phytophthora kernoviae
Phytophthora lagoariana Phytophthora lateralis
Phytophthora lavandula Phytophthora meadii
Phytophthora medicaginis Phytophthora megasperma
Phytophthora melonis Phytophthora nemorosa
Phytophthora nicotianae Phytophthora niederhauserii
Phytophthora palmivora Phytophthora pistaciae
Phytophthora porri Phytophthora pseudotsuga
Phytophthora quercina Phytophthora ramorum
Phytophthora richardiae Phytophthora sinensis
Phytophthora siskiyouensis Phytophthora sojae
Phytophthora syringae Phytophthora tropicalis
Phytophthora uliginosa

1The list above represents the Phytophthora species that have been shown to be detected by the Phytophthora genus ELISA test and does not represent all species that may be detected. If you have confirmed detection of a Phytophthora species not on this list, please contact us. We would like to work with you to further validate the Phytophthora genus ELISA detection capabilities.


Cross-reactions
Plasmopara viburni Pythium dissotocum2
Pythium irregulare2 Pythium myriotylum2
Pythium olegandrum2

2This test has not been validated for use as a tool for routine detection of Pythium and therefore is not recommended for such use.

For a list of validated hosts, please see the Full Validation Report.

Option Unit Purchase
PathoScreen® Kit, Phytophthora (Phyt), 96 testwells
PSA 92601/0096
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PathoScreen® Kit, Phytophthora (Phyt), 288 testwells
PSA 92601/0288
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PathoScreen® Kit, Phytophthora (Phyt), 480 testwells
PSA 92601/0480
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