ELISA Reagent Set for Phytophthora (Phyt)
The Phytophthora (Phyt) ELISA test system is intended for use with plant samples exhibiting symptoms of Phytophthora infection. This test can also be used to test fungal culture samples. The Phytophthora ELISA detects Phytophthora to the genus level and cannot differentiate species. For a full list of species that have experimentally detected, click here.
This test is the approved high throughput screening method in a U.S. national survey for Phytophthora ramorum, the causal agent of sudden oak death disease.
If you would prefer to add a field-deployable assay to your testing toolkit, please check out Agdia's Phyt ImmunoStrips®
Click here for other product documents such as the User Guide or Buffer Formulations.
Included:
- Capture antibody
- Alkaline phosphatase enzyme conjugate
- RUB6, 1X Ready to use
- General extract buffer 2, GEB2 powder
- 96-well microtiter plates, solid* (*strip in single plate set)
- User Guide
Required but Not Included:
- Buffer Pack (ACC 00113)
- Carbonate coating buffer, 10X concentrate
- PNP substrate buffer, 5X concentrate
- PNP substrate tablets, 5 mg each
- PBST buffer, powder
- Buffer Formulations from Chemicals
- Buffer Instructions from Powders and Concentrates
- Logsheet
For a full list of included items, including sizes, please see the Content List.
Validation Report Summary
Test Label: Alkaline Phosphatase |
Full Validation Report |
| Analytical Sensitivity |
|---|
| Limit of detection: 1:1,280 dilution of infected tissue (pathogen titer unknown) |
| Phytophthora Species Detected1 | |
|---|---|
| Phytophthora alticola-type | Phytophthora asparagi |
| Phytophthora alni | Phytophthora boehmeriae |
| Phytophthora bisheria | Phytophthora cactorum |
| Phytophthora cajani | Phytophthora cambivora |
| Phytophthora canlensis | Phytophthora capsici |
| Phytophthora cinnamomi var parvispora | Phytophthora cinnamomi var robiniae |
| Phytophthora citricola | Phytophthora citrophthora |
| Phytophthora cryptogea | Phytophthora drechsleri |
| Phytophthora europaea | Phytophthora erythroseptica |
| Phytophthora fragariae var fragariae | Phytophthora fragariae var rubi |
| Phytophthora glovera | Phytophthora gonapodyides |
| Phytophthora heveae | Phytophthora hibernalis |
| Phytophthora infestans | Phytophthora kernoviae |
| Phytophthora lagoariana | Phytophthora lateralis |
| Phytophthora lavandula | Phytophthora meadii |
| Phytophthora medicaginis | Phytophthora megasperma |
| Phytophthora melonis | Phytophthora nemorosa |
| Phytophthora nicotianae | Phytophthora niederhauserii |
| Phytophthora palmivora | Phytophthora pistaciae |
| Phytophthora porri | Phytophthora pseudotsuga |
| Phytophthora quercina | Phytophthora ramorum |
| Phytophthora richardiae | Phytophthora sinensis |
| Phytophthora siskiyouensis | Phytophthora sojae |
| Phytophthora syringae | Phytophthora tropicalis |
| Phytophthora uliginosa | |
1The list above represents the Phytophthora species that have been shown to be detected by the Phytophthora genus ELISA test and does not represent all species that may be detected. If you have confirmed detection of a Phytophthora species not on this list, please contact us. We would like to work with you to further validate the Phytophthora genus ELISA detection capabilities.
| Cross-reactions | |
|---|---|
| Phytopythium litorale | Plasmopara halstedii |
| Plasmopara viburni | Pythium dissotocum2 |
| Pythium irregulare2 | Pythium myriotylum2 |
| Pythium olegandrum2 | |
2This test has not been validated for use as a tool for routine detection of Pythium and therefore is not recommended for such use.
For a list of validated hosts, please see the Full Validation Report.
| Additional Documents | ||
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Reagent Set for Phytophthora (Phyt), 96 testwells
SRA 92601/0096
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Shown in cart
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Reagent Set for Phytophthora (Phyt), 500 testwells
SRA 92601/0500
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Reagent Set for Phytophthora (Phyt), 1000 testwells
SRA 92601/1000
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Shown in cart
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Reagent Set for Phytophthora (Phyt), 5000 testwells
SRA 92601/5000
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Shown in cart
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