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PathoScreen® Kit for Cucumber mosaic virus -subgroup II (CMV II)

Tech Note: Coating Antibody Information

This ELISA test is a qualitative serological assay for the detection of Cucumber mosaic virus - subgroup II (CMV II) in cucurbit leaves. CMV II is a member of the Cucumovirus genus known for their non-enveloped, spherical virus particles.

This test system is specific for CMV subgroup II (CMV-ToRS). It does not detect CMV subgroup I (CMV-DTL) or other cucumoviruses including Peanut stunt virus and Tomato aspermy virus. For more technical information click here.

If you would prefer to add a field-deployable assay to your testing toolkit, please check out Agdia's CMV ImmunoStrips® (Will detect both CMV I and CMV II).

Click here for other product documents such as the User Guide or Buffer Instructions.


  • Antibody-coated 96-well microtiter plates
  • Detection antibody, bottle A
  • Alkaline phosphatase enzyme conjugate, bottle B
  • ECI buffer, 5X concentrate
  • PNP substrate tablets, 5 mg each
  • PNP substrate buffer, 5X concentrate
  • Positive control
  • PBST buffer, 20X concentrate, 50 mL
  • TWEEN® 20
  • General extract buffer, GEB
  • User Guide
  • Tech Note

For a full list of included items, including sizes, please see the Content List.

TWEEN® is a registered trademark of Croda International PLC.

Validation Report Summary

Test Label: Alkaline Phosphatase
Test Format: Compound-ELISA
Capture Reagent: Polyclonal
Detection Reagent: Monoclonal

Full Validation Report

Analytical Sensitivity
Limit of detection: 1:90 dilution of infected tissue (pathogen titer unknown)

Isolate Names / Geographic Strain Locations
CMV ATCC® PV-549™ CMV-100 (New Zealand)
CMV-101 (New Zealand) CMV-107 (New Zealand)
CMV-110 (New Zealand) CMV-119 (New Zealand)
CMV-223 (New Zealand) CMV-88 (New Zealand)
CMV-90 (New Zealand) CMV-94 (New Zealand)
CMV-97 (New Zealand) CMV-98 (New Zealand)
CMV-C105 (MI, USA) CMV-C109 (CA, USA)
CMV-F354 seed transmissible (CA, USA) CMV-Kaper S (ATCC® PV-242™)
CMV-Mal1 (USA) CMV-Q (ATCC® PV-289™) (Australia)
CMV-Q (Australia) CMV-R (France)
CMV-R76B (England) CMV-Rm (France)
CMV-RS15 (Taiwan) CMV-S (South Africa)
CMV-T1T2Q3 CMV-U (Austraila)

None Known

For a list of validated hosts, please see the Full Validation Report.

Option Unit Purchase
PathoScreen® Kit, Cucumber mosaic virus subgroup II (CMV II), 96 testwells
PSA 44800/0096
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PathoScreen® Kit, Cucumber mosaic virus subgroup II (CMV II), 288 testwells
PSA 44800/0288
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PathoScreen® Kit, Cucumber mosaic virus subgroup II (CMV II), 480 testwells
PSA 44800/0480
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