PathoScreen® Kit for Cucumber mosaic virus -subgroup I (CMV I)

Tech Note: Coating Antibody Information

This ELISA test is a qualitative serological assay for the detection of Cucumber mosaic virus - subgroup I (CMV I) in cucurbit leaves. CMV I is a member of the Cucumovirus genus known for their non-enveloped, spherical virus particles.

This test system is specific for CMV subgroup I, (CMV-DTL). It does not detect CMV subgroup II (CMV-ToRS) or other cucumoviruses including Peanut stunt virus and Tomato aspermy virus. For more technical information click here

If you would prefer to add a field-deployable assay to your testing toolkit, please check out Agdia's CMV ImmunoStrips® (Will detect both CMV I and CMV II)

Click here for other product documents such as the User Guide or Buffer Instructions.

Included:

  • Antibody-coated 96-well microtiter plates
  • Detection antibody, bottle A
  • Alkaline phosphatase enzyme conjugate, bottle B
  • ECI buffer, 5X concentrate
  • PNP substrate tablets, 5 mg each
  • PNP substrate buffer, 5X concentrate
  • Synthetic positive control
  • PBST buffer, 20X concentrate, 50 mL
  • TWEEN® 20
  • General extract buffer, GEB
  • User Guide
  • Tech Note

For a full list of included items, including sizes, please see the Content List.

TWEEN® is a registered trademark of Croda International PLC.

Validation Report Summary

Test Label: Alkaline Phosphatase
Test Format: Compound-ELISA
Capture Reagent: Polyclonal
Detection Reagent: Monoclonal

Full Validation Report


Analytical Sensitivity
Limit of detection: 1:3,038 dilution of infected tissue (pathogen titer unknown)

Isolate Names / Geographic Strain Locations
CMV-1 (USA) CMV-16 (Taiwan)
CMV-1A1 (IL, USA) CMV-2A1 (IL, USA)
CMV-Ala1 (AL, USA) CMV-Ala3 (AL, USA)
CMV-ATCC PV-29 (WI, USA) CMV-ATCC PV-30 (MD, USA)
CMV-ATCC PV-453 (CA, USA) CMV-ATCC PV-539 (CA, USA)
CMV-ATCC PV-540 (Japan) CMV-ATCC PV-543 (Japan)
CMV-ATCC PV-544 (NJ, USA) CMV-ATCC PV-545 (NJ, USA)
CMV-ATCC PV-546 (United Kingdoms) CMV-ATCC PV-547 (NY, USA)
CMV-ATCC PV-548 (NY, USA) CMV-ATCC PV-59 (AZ, USA)
CMV-BCM (Taiwan) CMV-C105 (MI, USA)
CMV-C107 (IN, USA) CMV-C109 (CA, USA)
CMV-C297 (AZ, USA) CMV-C310 (N890) (GA, USA)
CMV-D (France) CMV-F10 (Taiwan)
CMV-Fny (NY, USA) CMV-ICS (Indonesia)
CMV-Ixora (Philippines) CMV-M48 (Taiwan)
CMV-Mal1 (USA) CMV-N-1A (NY, USA)
CMV-N-1B (NY, USA) CMV-NT4 (Taiwan)
CMV-NT9 (Taiwan) CMV-O (Australia)
CMV-O69 (FL, USA) CMV-P1018 (Taiwan)
CMV-P117 (Taiwan) CMV-P1347 (Taiwan)
CMV-P1360 (Taiwan) CMV-P1369 (Taiwan)
CMV-P1370 (Taiwan) CMV-P1441 (Taiwan)
CMV-P1471 (Taiwan) CMV-P1624 (Taiwan)
CMV-P1628 (Taiwan) CMV-P184 (Taiwan)
CMV-P1886 (Taiwan) CMV-P1927 (Taiwan)
CMV-P1929 (Taiwan) CMV-P212 (Taiwan)
CMV-P2469 (Taiwan) CMV-P2853 (Taiwan)
CMV-P2874 (Taiwan) CMV-P2875 (Taiwan)
CMV-P2878 (Taiwan) CMV-P3 (Taiwan)
CMV-P427 (Taiwan) CMV-P522 (Taiwan)
CMV-P600 (Taiwan) CMV-P652 (Taiwan)
CMV-P661 (Taiwan) CMV-P888 (Taiwan)
CMV-P922 CMV-P959 (Taiwan)
CMV-Peet (Taiwan) CMV-RS12 (Taiwan)
CMV-S2A (NY, USA) CMV-Sny (NY, USA)
CMV-T (Austrailia) CMV-T118 (Japan)
CMV-T2373 (Taiwan) CMV-T2374 (Taiwan)
CMV-V135 CMV-V389 (Italy)
CMV-WT (WI, USA) CMV-Y (Japan)

Cross-reactions
None Known

For a list of validated hosts, please see the Full Validation Report.

Option Unit Purchase
PathoScreen® Kit, Cucumber mosaic virus subgroup I (CMV I), 96 testwells
PSA 44700/0096
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PathoScreen® Kit, Cucumber mosaic virus subgroup I (CMV I), 288 testwells
PSA 44700/0288
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PathoScreen® Kit, Cucumber mosaic virus subgroup I (CMV I), 480 testwells
PSA 44700/0480
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