PathoScreen® Kit for Cucumber green mottle mosaic virus (CGMMV)

Tech Note: Alternate Seed Protocol

This ELISA test is a qualitative serological assay for the detection of Cucumber green mottle mosaic virus (CGMMV), the causal agent of Cucumber green mottle mosaic disease, in cucrubit crops including cucumber, melon, and watermelon leaves and seeds. CGMMV is a member of the Tobamovirus genus known for their rod-shaped virus particles.

Cucumber green mottle mosaic is an extremely stable virus and can easily infect adjacent plants and contaminate all kinds of surfaces, such as benches, pots, and tools.

If you would prefer to add a field-deployable assay to your testing toolkit, please check out Agdia's CGMMV ImmunoStrips®

Click here for other product documents such as the User Guide or Buffer Instructions.

Included:

Required but Not Included:

  • Antibody-coated 96-well microtiter plates
  • Detection antibody, bottle A
  • Alkaline phosphatase enzyme conjugate, bottle B
  • ECI buffer, 5X concentrate
  • PNP substrate tablets, 5 mg each
  • PNP substrate buffer, 5X concentrate
  • Synthetic positive control
  • PBST buffer, 20X concentrate, 50 mL
  • TWEEN® 20
  • General extract buffer, GEB
  • User Guide
  • Tech Note

For a full list of included items, including sizes, please see the Content List.

TWEEN® is a registered trademark of Croda International PLC.

Validation Report Summary

Test Label: Alkaline Phosphatase
Test Format: Compound Double Antibody Sandwich (TAS) ELISA
Capture Reagent: Polyclonal
Detection Reagent: Monoclonal

Full Validation Report


Analytical Sensitivity
Limit of detection: 1:194,400 dilution of infected tissue (pathogen titer unknown)

Isolate Names / Geographic Strain Locations
CGMMV ATCC-391 (Japan) CGMMV Bulgaria isolate
CGMMV Canada isolate CGMMV France isolate
CGMMV Greece isolate CGMMV Israel isolate
CGMMV Kuwait isolate CGMMV Latvia isolate
CGMMV Netherlands isolate CGMMV Russia isolate
CGMMV USA isolate

Cross-reactions
None Known

For a list of validated hosts, please see the Full Validation Report.

Option Unit Purchase
PathoScreen® Kit for Cucumber green mottle mosaic virus (CGMMV), 96 testwells
PSA 45702/0096
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PathoScreen® Kit for Cucumber green mottle mosaic virus (CGMMV), 288 testwells
PSA 45702/0288
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PathoScreen® Kit for Cucumber green mottle mosaic virus (CGMMV), 480 testwells
PSA 45702/0480
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