PathoScreen® Kit for Cucumber green mottle mosaic virus (CGMMV)
Tech Note: Alternate Seed Protocol
This ELISA test is a qualitative serological assay for the detection of Cucumber green mottle mosaic virus (CGMMV), the causal agent of Cucumber green mottle mosaic disease, in cucrubit crops including cucumber, melon, and watermelon leaves and seeds. CGMMV is a member of the Tobamovirus genus known for their rod-shaped virus particles.
Cucumber green mottle mosaic is an extremely stable virus and can easily infect adjacent plants and contaminate all kinds of surfaces, such as benches, pots, and tools.
If you would prefer to add a field-deployable assay to your testing toolkit, please check out Agdia's CGMMV ImmunoStrips®
Click here for other product documents such as the User Guide or Buffer Instructions.
- Antibody-coated 96-well microtiter plates
- Detection antibody, bottle A
- Alkaline phosphatase enzyme conjugate, bottle B
- ECI buffer, 5X concentrate
- PNP substrate tablets, 5 mg each
- PNP substrate buffer, 5X concentrate
- Synthetic positive control
- PBST buffer, 20X concentrate, 50 mL
- TWEEN® 20
- General extract buffer, GEB
- User Guide
- Tech Note
Required but Not Included:
For a full list of included items, including sizes, please see the Content List.
TWEEN® is a registered trademark of Croda International PLC.
Validation Report Summary
Test Label: Alkaline Phosphatase
Full Validation Report
|Limit of detection: 1:194,400 dilution of infected tissue (pathogen titer unknown)|
|Isolate Names / Geographic Strain Locations|
|CGMMV ATCC® PV-391™ (Japan)||CGMMV-CG001 (CA, USA)|
|CGMMV-CG002 (CA, USA)||CGMMV-CG003 (CA, USA)|
|CGMMV-CG004 (CA, USA)||CGMMV-CG006 (Israel)|
|CGMMV-CG007 (Netherlands)||CGMMV-CG008 (Netherlands)|
|CGMMV-CG009 (Netherlands)||CGMMV-CG010 (Netherlands)|
|CGMMV-CG011 (Netherlands)||CGMMV-CG012 (Netherlands)|
|CGMMV-CG013 (Netherlands)||CGMMV-CG014 (Netherlands)|
|CGMMV-CG015 (Netherlands)||CGMMV-CG019 (Thailand)|
|CGMMV-CG020 (Netherlands)||CGMMV-CG021 (Netherlands)|
|CGMMV-CG022 (Netherlands)||CGMMV-CG023 (Netherlands)|
|CGMMV-CG025 (Latvia)||CGMMV-CG026 (France)|
|CGMMV-CG027 (France)||CGMMV-CG028 (Bulgaria)|
|CGMMV-CG029 (Greece)||CGMMV-CG030 (Greece)|
|CGMMV-CG031 (Netherlands)||CGMMV-CG032 (Russia)|
|CGMMV-CG033 (Netherlands)||CGMMV-CG034 (Netherlands)|
|CGMMV-CG035 (Kuwait)||CGMMV-CG036 (Canada)|
For a list of validated hosts, please see the Full Validation Report.
PathoScreen® Kit for Cucumber green mottle mosaic virus (CGMMV), 96 testwells
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