ELISA Reagent Set for Cucumber green mottle mosaic virus (CGMMV)

Tech Note: Alternate Seed Protocol

This ELISA test is a qualitative serological assay for the detection of Cucumber green mottle mosaic virus (CGMMV), the causal agent of Cucumber green mottle mosaic disease, in cucrubit crops including cucumber, melon, and watermelon leaves and seeds. CGMMV is a member of the Tobamovirus genus known for their rod-shaped virus particles.

Cucumber green mottle mosaic is an extremely stable virus and can easily infect adjacent plants and contaminate all kinds of surfaces, such as benches, pots, and tools.

If you would prefer to add a field-deployable assay to your testing toolkit, please check out Agdia's CGMMV ImmunoStrips®

Click here for other product documents such as the User Guide or Buffer Formulations.

Included:

  • Capture antibody
  • Detection antibody, bottle A
  • Alkaline phosphatase enzyme conjugate, bottle B
  • 96-well microtiter plates, solid* (*strip in single plate set)
  • User Guide
  • Tech Note

Required but Not Included:

For a full list of included items, including sizes, please see the Content List.

TWEEN® is a registered trademark of Croda International PLC.

Validation Report Summary

Test Label: Alkaline Phosphatase
Test Format: Compound Double Antibody Sandwich (TAS) ELISA
Capture Reagent: Polyclonal
Detection Reagent: Monoclonal

Full Validation Report


Analytical Sensitivity
Limit of detection: 1:194,400 dilution of infected tissue (pathogen titer unknown)

Isolate Names / Geographic Strain Locations
CGMMV ATCC® PV-391™ (Japan) CGMMV-CG001 (CA, USA)
CGMMV-CG002 (CA, USA) CGMMV-CG003 (CA, USA)
CGMMV-CG004 (CA, USA) CGMMV-CG006 (Israel)
CGMMV-CG007 (Netherlands) CGMMV-CG008 (Netherlands)
CGMMV-CG009 (Netherlands) CGMMV-CG010 (Netherlands)
CGMMV-CG011 (Netherlands) CGMMV-CG012 (Netherlands)
CGMMV-CG013 (Netherlands) CGMMV-CG014 (Netherlands)
CGMMV-CG015 (Netherlands) CGMMV-CG019 (Thailand)
CGMMV-CG020 (Netherlands) CGMMV-CG021 (Netherlands)
CGMMV-CG022 (Netherlands) CGMMV-CG023 (Netherlands)
CGMMV-CG025 (Latvia) CGMMV-CG026 (France)
CGMMV-CG027 (France) CGMMV-CG028 (Bulgaria)
CGMMV-CG029 (Greece) CGMMV-CG030 (Greece)
CGMMV-CG031 (Netherlands) CGMMV-CG032 (Russia)
CGMMV-CG033 (Netherlands) CGMMV-CG034 (Netherlands)
CGMMV-CG035 (Kuwait) CGMMV-CG036 (Canada)
CGMMV-CG038 (China)

Cross-reactions
None Known

For a list of validated hosts, please see the Full Validation Report.

Option Unit Purchase
Reagent Set for Cucumber green mottle mosaic virus (CGMMV), 96 testwells
SRA 45702/0096
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Reagent Set for Cucumber green mottle mosaic virus (CGMMV), 500 testwells
SRA 45702/0500
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Reagent Set for Cucumber green mottle mosaic virus (CGMMV), 1000 testwells
SRA 45702/1000
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Reagent Set for Cucumber green mottle mosaic virus (CGMMV), 5000 testwells
SRA 45702/5000
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