PathoScreen® Kit for Arabis mosaic virus (ArMV)

Tech Note: Sample Extraction Buffer Change

Alternate Protocol: Alternate Extraction Buffer

This ELISA test is a qualitative serological assay for the detection of Arabis mosaic virus (ArMV), the causal agent of Raspberry yellow dwarf disease, in crops including grapevines, hops, raspberry, and strawberry, as well as other fruit, vegetable, and ornamentals. ArMV is a member of the Nepovirus genus known for their transmission by nematodes.

If you would prefer to add a field-deployable assay to your testing toolkit, please check out Agdia's ArMV ImmunoStrips®

Click here for other product documents such as the User Guide or Buffer Instructions.

Included:

  • Antibody-coated 96-well microtiter plates
  • Alkaline phosphatase enzyme conjugate
  • RUB6 enzyme conjugate diluent, 1X ready to use
  • PNP substrate tablets, 5 mg each
  • PNP substrate buffer, 5X concentrate
  • Positive control
  • PBST buffer, 20X concentrate, 50 mL
  • TWEEN® 20
  • General extract buffer, GEB
  • User Guide
  • Tech Note

Required but Not Included:

For a full list of included items, including sizes, please see the Content List.

TWEEN® is a registered trademark of Croda International PLC.

Validation Report Summary

Test Label: Alkaline Phosphatase
Test Format: Double Antibody Sandwich (DAS) ELISA
Capture Reagent: Polyclonal
Detection Reagent: Polyclonal

Full Validation Report


Analytical Sensitivity
Limit of detection: 1:4,860 dilution of infected tissue (pathogen titer unknown)

Isolate Names / Geographic Strain Locations
ArMV PV-0045 ArMV PV-0046
ArMV PV-0215 ArMV PV-0216 (Denmark)
ArMV Europe isolate ArMV USA isolate

Cross-reactions
None Known

For a list of validated hosts, please see the Full Validation Report.

Option Unit Purchase
PathoScreen® Kit, Arabis mosaic virus (ArMV), 96 testwells
PSA 23203/0096
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PathoScreen® Kit, Arabis mosaic virus (ArMV), 288 testwells
PSA 23203/0288
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PathoScreen® Kit, Arabis mosaic virus (ArMV), 480 testwells
PSA 23203/0480
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