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ELISA Reagent Set for ArMV (Arabis mosaic virus)

Alternate Protocol: Alternate Extraction Buffer

This ELISA test is a qualitative serological assay for the detection of Arabis mosaic virus (ArMV), the causal agent of Raspberry yellow dwarf disease, in crops including grapevines, hops, raspberry, and strawberry, as well as other fruit, vegetable, and ornamentals. ArMV is a member of the Nepovirus genus known for their transmission by nematodes.

If you would prefer to add a field-deployable assay to your testing toolkit, please check out Agdia's ArMV ImmunoStrips®

Click here for other product documents such as the Reagent Set User Guide or PathoScreen Kit User Guide, click on the Documents tab for the full list.

See below for a summary of our ELISA format offerings. Not all formats are available for every test. See the Included Items tab for full details.

  • Reagent Set: Includes coating antibody, enzyme conjugate, and uncoated 96-well plate(s). Popular choice for established high-throughput laboratories.
  • PathoScreen Complete Kit: Includes pre-coated 96-well plate(s), enzyme conjugate, positive control, and all required buffers. Easiest format to get started with ELISA and requires the least amount of time and labor to run.
  • Capture Antibody: Only includes capture antibody. This is a partial component not usually ordered by itself. Unavailable on website. Call or email us to order.
  • Enzyme Conjugate: Includes enzyme conjugate and 96-well plate(s). This is a partial component not usually ordered by itself. Unavailable on website. Call or email us to order.

Reagent Set

Included in Reagent Set:

  • Capture antibody
  • Alkaline phosphatase enzyme conjugate
  • RUB6 enzyme conjugate diluent, 1X ready to use
  • 96-well microtiter plates, solid* (*strip in single plate set)
  • Reagent Set User Guide

Required but Not Included:

PathoScreen Kit

Included in PathoScreen Kit:

  • Antibody-coated 96-well microtiter plates
  • Alkaline phosphatase enzyme conjugate
  • RUB6 enzyme conjugate diluent, 1X ready to use
  • PNP substrate tablets, 5 mg each
  • PNP substrate buffer, 5X concentrate
  • Positive control
  • PBST buffer, 20X concentrate, 50 mL
  • TWEEN® 20
  • General extract buffer, GEB
  • PathoScreen User Guide

Coating Antibody

Included in Coating Antibody:

  • Capture antibody
  • 96-well microtiter plates, solid

Required but Not Included:

  • Only a partial component; see above for complete options

Enzyme Conjugate

Included in Enzyme Conjugate:

Required but Not Included:

  • Only a partial component; see above for complete options

For a full list of included items, including sizes, please see the Content List.

TWEEN® is a registered trademark of Croda International PLC.

Validation Report Summary

Test Label: Alkaline Phosphatase
Test Format: Double Antibody Sandwich (DAS) ELISA
Capture Reagent: Polyclonal
Detection Reagent: Polyclonal

Full Validation Report


Analytical Sensitivity
Limit of detection: 1:4,860 dilution of infected tissue (pathogen titer unknown)

Isolate Names / Geographic Strain Locations
ArMV PV-0045 ArMV PV-0046
ArMV PV-0215 ArMV PV-0216 (Denmark)
ArMV Europe isolate ArMV USA isolate

Cross-reactions
None Known

For a list of validated hosts, please see the Full Validation Report.

Option Unit Purchase
ArMV Reagent Set, 96 Testwells
SRA 23203/0096
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ArMV Reagent Set, 500 Testwells
SRA 23203/0500
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ArMV Reagent Set, 1000 Testwells
SRA 23203/1000
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ArMV Reagent Set, 5000 Testwells
SRA 23203/5000
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ArMV PathoScreen® Complete Kit, 96 Testwells
PSA 23203/0096
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ArMV PathoScreen® Complete Kit, 288 Testwells
PSA 23203/0288
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ArMV PathoScreen® Complete Kit, 480 Testwells
PSA 23203/0480
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