AmplifyRP® XRT for GLRaV-3 (Grapevine leafroll-associated virus 3)
Test Label: FAM-labeled target probe
Test Format: AmplifyRP® XRT
Internal Control: Yes - Endogenous
AmplifyRP® XRT for GLRaV-3 is a rapid RNA amplification and detection platform designed for testing grapevines for Grapevine leafroll-associated virus 3. This kit includes lyophilized reaction pellets containing the necessary reagents to amplify GLRaV-3 RNA and an endogenous RNA control at a single operating temperature (42 °C).
AmplifyRP® XRT is a real-time isothermal nucleic acid amplification and detection system that rapidly amplifies small portions of DNA or RNA and offers unrivaled detection capabilities in an easy-to-use testing format. It offers comparable sensitivity and specificity to published PCR methods while eliminating laborious and costly nucleic acid extractions.
The test can be performed virtually anywhere using the battery operated AmpliFire® fluorometer. Assay parameters are loaded via barcode and results are automatically displayed as ( + ) or ( - ).
Prior molecular diagnostic experience is not required to perform AmplifyRP® XRT tests.
NOTE: This assay requires a fluorometer to work properly.
If you need a printable barcode for this assay, click here.
Limit of Detection (LOD): 2.5 fg RNA transcript per reaction
- Inclusive: Designed to detect all GLRaV-3 isolates.
- Exclusive: Does not detect: Arabis mosaic virus (ArMV), Botrytis cinerea, Grapevine fanleaf virus (GFLV), Grapevine fleck virus (GFkV), Grapevine leafroll-associated virus 1 (GLRaV-1), Grapevine leafroll-associated virus 2 (GLRaV-2), Grapevine leafroll-associated virus 4 (GLRaV-4), Grapevine leafroll-associated virus 7 (GLRaV-7), Grapevine leafroll-associated virus 10 (GLRaV-10), Grapevine pinot gris virus (GPGV), Grapevine red blotch virus (GRBV), Little cherry virus 2 (LChV2), Phytoplasma solani, Phytoplasma vitis, Tobacco ringspot virus (TRSV), Tomato ringspot virus (ToRSV), and Xylella fastidiosa (Xf)
- Predicted not to detect via insilico data: Grapevine leafroll-associated virus 5 (GLRaV-5), Grapevine leafroll-associated virus 6 (GLRaV-6), Grapevine leafroll-associated virus 9 (GLRaV-9), Grapevine leafroll-associated virus Car (GLRaV-Car), Grapevine leafroll-associated virus De (GLRaV-De), Grapevine leafroll-associated virus Pr (GLRaV-Pr), Peach rosette mosaic virus (PRMV), Pineapple mealybug wilt associated virus 1 (PMWaV-1), Pineapple mealybug wilt associated virus 2 (PMWaV-2), Pineapple mealybug wilt associated virus 3 (PMWaV-3), Plum bark necrosis stem pitting-associated virus (PBNSPaV), and Strawberry latent ringspot virus (SLRSV)
Host Limitations: None
Total Assay Time: Less than 30 minutes when used with the AmpliFire® as a real-time assay.