AmplifyRP® XRT for Phytoplasma solani BN (Bois Noir)
AmplifyRP® XRT for BN is a rapid DNA amplification and detection platform designed for testing grapevines for Phytoplasma solani Bois Noir. This kit includes lyophilized reaction pellets containing the necessary reagents to amplify BN DNA and an endogenous DNA control at a single operating temperature (42 °C).
AmplifyRP® XRT is a real-time isothermal nucleic acid amplification and detection system that rapidly amplifies small portions of DNA or RNA and offers unrivaled detection capabilities in an easy-to-use testing format. It offers comparable sensitivity and specificity to published PCR methods while eliminating laborious and costly nucleic acid extractions.
The test can be performed virtually anywhere using the battery operated AmpliFire® fluorometer. Assay parameters are loaded via barcode and results are automatically displayed as ( + ) or ( - ). NOTE: This assay requires a fluorometer to work properly.
Prior molecular diagnostic experience is not required to perform AmplifyRP® XRT tests. Total assay time is less than 30 minutes when used with the AmpliFire® as a real-time assay.
Click here for other product documents such as the User Guide or printable barcode.
Included:
- XRT reaction pellets for BN
- Pre-filled 100 µL PD1 Pellet diluent tubes
- GEB-filled mesh extraction bags
- User Guide
Required but Not Included:
- 5 µL Pipette
- 25 µL Pipette
- 5 and 25 µL Barrier Pipette Tips
- AmpliFire® or equivalent fluorometer
Optional:
For a full list of included items, including sizes, please see the Content List.
Validation Report Summary
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Test Format: AmplifyRP® XRT |
Full Validation Report |
| Analytical Sensitivity | |
|---|---|
| Analytical Sensitivity: | The assay is 66.7% sensitive between 50 zg/µL and 500 zg/µL. (n=8) |
| Limit of detection: | The assay has a 100% detection rate at 500 zg/µL with DNA fragment. (n=5) |
| The assay has a 33.3% detection rate at 50 zg/µL with DNA fragment. (n=3) | |
| Isolate Names / Geographic Strain Locations | |
|---|---|
| BN-231/091 | BN-284/091 |
| BN-Portugal1 (Portugal)1 | BN-SBR1 |
| BN-StC (France) | BN-Stol C1 |
| BN-Stol111 | BN-Stol11_1_C1 |
| BN-Stol11_2_U1 | BN-Stol11_3_C1 |
| BN-Stol11-Conv12/2011-Bg (Bulgaria)1 | BN-Stol11-Conv2/2010-Bg (Bulgaria)1 |
| BN-Stol11-Rubus1/2010-Bg (Bulgaria)1 | BN-STOL2 (Serbia)1 |
| BN-STOL3 (Serbia)1 | |
1Predicted detection by in silico analysis only
| Cross-reactions | |
|---|---|
| Virus Name | Species Name |
| None Known | |
For a list of validated hosts, please see the Full Validation Report.
| Additional Documents | ||
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