AmplifyRP® XRT for Phytoplasma solani BN (Bois Noir)
Test Label: Biotin labeled primer / FAM probe
Test Format: AmplifyRP® XRT
Internal Control: Yes - Endogenous
AmplifyRP XRT for BN is a rapid DNA amplification and detection platform designed for testing grapevines for Phytoplasma solani Bois Noir. This kit includes lyophilized reaction pellets containing the necessary reagents to amplify BN DNA and an endogenous DNA control at a single operating temperature (42 °C).
AmplifyRP XRT is a real-time isothermal DNA amplification and detection system that rapidly amplifies small portions of DNA and offers unrivaled detection capabilities in an easy-to-use testing format. It offers comparable sensitivity and specificity to published PCR methods while eliminating laborious and costly nucleic acid extractions.
The test can be performed virtually anywhere using the battery operated AmpliFire fluorometer. Assay parameters are loaded via barcode and results are automatically displayed as ( + ) or ( - ).
Prior molecular diagnostic experience is not required to perform AmplifyRP XRT tests.
NOTE: This assay requires a fluorometer to work properly.
If you need a printable barcode for this assay, click here.
Limit of Detection (LOD): ~ 50 genomic copies per reaction
- Inclusive: Designed to detect all BN and Candidatus Phytoplasma solani isolates.
- Does not detect: Phytoplasma vitis.
- Predicted not to detect: Phytoplasma fragariae, Phytoplasma australiense, Phytoplasma asteris, Phytoplasma pruni, Phytoplasma americanum, Phytoplasma mali, Phytoplasma pyri, Phytoplasma japonicum, Phytoplasma convolvuli, or Phytoplasma prunorum.
Total Assay Time: Less than 30 minutes when used with the AmpliFire as a real-time assay.