ELISA Reagent Set for Tomato leaf curl New Delhi virus (ToLCNDV) - Cucurbit

Host reaction: This test is recommended for use on cucurbit tissues only. Non-specific reactions have been observed with other plant species.

This ELISA test is a qualitative serological assay for the detection of Tomato leaf curl New Delhi virus (ToLCNDV) in cucurbit leaves. ToLCNDV is a member of the Begomovirus genus known for their transmission by whiteflies. ToLCNDV has been reported in European and Asian growing regions and is on the EPPO Alert list.

If you would prefer to add a field-deployable assay to your testing toolkit, please check out Agdia's ToLCNDV ImmunoStrips®

Click here for other product documents such as the User Guide or Buffer Formulations.

Included:

  • Capture antibody
  • Alkaline phosphatase enzyme conjugate
  • 96-well microtiter plates, solid* (*strip in single plate set)
  • User Guide

Required but Not Included:

For a full list of included items, including sizes, please see the Content List.

TWEEN® is a registered trademark of Croda International PLC.

Validation Report Summary

Test Label: Alkaline Phosphatase
Test Format: Double Antibody Sandwich (DAS) ELISA
Capture Reagent: Polyclonal
Detection Reagent: Polyclonal

Full Validation Report


Analytical Sensitivity
Limit of detection: 1:2,430 dilution of infected tissue (pathogen titer unknown)

Isolate Names / Geographic Strain Locations
This assay was designed to detect all strains and isolates of ToLCNDV. Fourteen distinct samples of ToLCNDV have been experimentally proven to be detected including samples of PV-1109 (Spain) and PV-1111 (Spain).

Cross-reactions
African cassava mosaic virus (ACMV) Bean golden mosaic virus (BGMV)
Squash leaf curl virus (SLCV) Tomato yellow leaf curl virus (TYLCV)1

1One out of four samples gave a low level positive reaction. High titer samples may cross react.

For a list of validated hosts, please see the Full Validation Report.

Option Unit Purchase
Reagent Set for ToLCNDV-Cucurbit, 96 testwells
SRA 38900/0096
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Reagent Set for ToLCNDV-Cucurbit, 500 testwells
SRA 38900/0500
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Reagent Set for ToLCNDV-Cucurbit, 1000 testwells
SRA 38900/1000
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Reagent Set for ToLCNDV-Cucurbit, 5000 testwells
SRA 38900/5000
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