ELISA Reagent Set for Tomato leaf curl New Delhi virus (ToLCNDV) - Cucurbit
Host reaction: This test is recommended for use on cucurbit tissues only. Non-specific reactions have been observed with other plant species.
This ELISA test is a qualitative serological assay for the detection of Tomato leaf curl New Delhi virus (ToLCNDV) in cucurbit leaves. ToLCNDV is a member of the Begomovirus genus known for their transmission by whiteflies. ToLCNDV has been reported in European and Asian growing regions and is on the EPPO Alert list.
If you would prefer to add a field-deployable assay to your testing toolkit, please check out Agdia's ToLCNDV ImmunoStrips®
Click here for other product documents such as the User Guide or Buffer Formulations.
Included:
- Capture antibody
- Alkaline phosphatase enzyme conjugate
- 96-well microtiter plates, solid* (*strip in single plate set)
- User Guide
Required but Not Included:
- Buffer Pack (ACC 00111)
- Carbonate coating buffer, 10X concentrate
- PNP substrate buffer, 5X concentrate
- PNP substrate tablets, 5 mg each
- PBST buffer, powder
- ECI buffer, 5X concentrate
- General extract buffer
- TWEEN® 20
- Buffer Formulations from Chemicals
- Logsheet
For a full list of included items, including sizes, please see the Content List.
TWEEN® is a registered trademark of Croda International PLC.
Validation Report Summary
Test Label: Alkaline Phosphatase |
Full Validation Report |
| Analytical Sensitivity |
|---|
| Limit of detection: 1:2,430 dilution of infected tissue (pathogen titer unknown) |
| Isolate Names / Geographic Strain Locations | |
|---|---|
| This assay was designed to detect all strains and isolates of ToLCNDV. Fourteen distinct samples of ToLCNDV have been experimentally proven to be detected including samples of PV-1109 (Spain) and PV-1111 (Spain). | |
| Cross-reactions | |
|---|---|
| African cassava mosaic virus (ACMV) | Bean golden mosaic virus (BGMV) |
| Cucurbit leaf crumple virus (CuLCrV) | Squash leaf curl virus (SLCV) |
| Tomato yellow leaf curl virus (TYLCV)1 | |
1One out of four samples gave a low level positive reaction. High titer samples may cross react.
For a list of validated hosts, please see the Full Validation Report.
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| Option | Unit | Purchase |
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Reagent Set for ToLCNDV-Cucurbit, 96 testwells
SRA 38900/0096
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Shown in cart
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Reagent Set for ToLCNDV-Cucurbit, 500 testwells
SRA 38900/0500
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Shown in cart
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Reagent Set for ToLCNDV-Cucurbit, 1000 testwells
SRA 38900/1000
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Shown in cart
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Reagent Set for ToLCNDV-Cucurbit, 5000 testwells
SRA 38900/5000
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Shown in cart
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