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ELISA Reagent Set for Peanut stunt virus (PSV)

This ELISA test is a qualitative serological assay for the detection of Peanut stunt virus (PSV), also known as groundnut stunt, in bean and peanut leaves and seed. PVS is a member of the Cucumovirus genus known for their non-enveloped, spherical virus particles.

Peanut stunt virus causes severe disease in beans and peanuts. It can also infect a large number of other crops, including alfalfa, clover, cucurbits, tobacco, and tomato.

The aphids, Myzus persicae, Aphis craccivora, and Aphis spiraecola can all transmit PSV in a non-persistent manner. These aphid vectors can spread PSV to highly susceptible crops from reservoir host legumes such as alfalfa and clover, where it can overwinter. PSV can also be seed-transmitted.

This test system has very low background and no cross-reaction with Cucumber mosaic virus; for other technical information click here.. Using this test system, you can detect both the eastern strain and the western strain of PSV. Optical density values for samples infected with the eastern strain may be somewhat higher than for samples infected with the western strain.

Click here for other product documents such as the User Guide or Buffer Formulations.


  • Capture antibody
  • Alkaline phosphatase enzyme conjugate
  • 96-well microtiter plates, solid* (*strip in single plate set)
  • User Guide

Required but Not Included:

For a full list of included items, including sizes, please see the Content List.

TWEEN® is a registered trademark of Croda International PLC.

Validation Report Summary

Test Label: Alkaline Phosphatase
Test Format: Double Antibody Sandwich (DAS) ELISA
Capture Reagent: Polyclonal
Detection Reagent: Polyclonal

Full Validation Report

Analytical Sensitivity
Limit of detection: 1:32,400 dilution of infected tissue (pathogen titer unknown)

Isolate Names / Geographic Strain Locations
This assay was designed to detect all strains and isolates of PSV. Twenty-five distinct samples of PSV have been experimentally proven to be detected including samples of the Eastern and Western strains.

None Known

For a list of validated hosts, please see the Full Validation Report.

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Reagent Set for Peanut stunt virus (PSV), 96 testwells
SRA 44600/0096
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Reagent Set for Peanut stunt virus (PSV), 500 testwells
SRA 44600/0500
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Reagent Set for Peanut stunt virus (PSV), 1000 testwells
SRA 44600/1000
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Reagent Set for Peanut stunt virus (PSV), 5000 testwells
SRA 44600/5000
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