ELISA Test Kits for Pstew (Pantoea stewartii)
This ELISA test is a qualitative serological assay for the detection of Pantoea stewartii subsp. stewartii (Pstew), the causal agent of Stewart's Wilt, in corn leaves and seed. Agdia's ELISA test for Pstew provides an effective, lab-based method for phytosanitary assessment.
If you would prefer to add a field-deployable assay to your testing toolkit, please check out Agdia's Pstew ImmunoStrips®
Click here for other product documents such as the Reagent Set User Guide or PathoScreen Kit User Guide, click on the Documents tab for the full list.
See below for a summary of our ELISA format offerings. Not all formats are available for every test. See the Included Items tab for full details.
- Reagent Set: Includes coating antibody, enzyme conjugate, and uncoated 96-well plate(s). Popular choice for established high-throughput laboratories.
- PathoScreen Complete Kit: Includes pre-coated 96-well plate(s), enzyme conjugate, positive control, and all required buffers. Easiest format to get started with ELISA and requires the least amount of time and labor to run.
- Capture Antibody: Only includes capture antibody. This is a partial component not usually ordered by itself. Unavailable on website. Call or email us to order.
- Enzyme Conjugate: Includes enzyme conjugate and 96-well plate(s). This is a partial component not usually ordered by itself. Unavailable on website. Call or email us to order.
Reagent Set
Included in Reagent Set:
- Capture antibody
- Detection antibody, bottle A
- Alkaline phosphatase enzyme conjugate, bottle B
- 96-well microtiter plates, solid* (*strip in single plate set)
- Reagent Set User Guide
Required but Not Included:
- Buffer Pack (ACC 00111)
- Carbonate coating buffer, 10X concentrate
- PNP substrate buffer, 5X concentrate
- PNP substrate tablets, 5 mg each
- PBST buffer, powder
- ECI buffer, 5X concentrate
- General extract buffer
- TWEEN® 20
- Buffer Formulations from Chemicals
- Logsheet
PathoScreen Kit (Format Not Available)
Coating Antibody
Included in Coating Antibody:
- Capture antibody
- 96-well microtiter plates, solid
Required but Not Included:
- Only a partial component; see above for complete options
Enzyme Conjugate
Included in Enzyme Conjugate:
- Detection antibody, bottle A
- Alkaline phosphatase enzyme conjugate, bottle B
- Reagent Set User Guide
Required but Not Included:
- Only a partial component; see above for complete options
For a full list of included items, including sizes, please see the Content List.
TWEEN® is a registered trademark of Croda International PLC.
Validation Report Summary
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Test Label: Alkaline Phosphatase |
Full Validation Report |
| Analytical Sensitivity |
|---|
| Limit of detection: 5.1x105 CFU/mL |
| Isolate Names / Geographic Strain Locations | |
|---|---|
| Pstew-1303 | Pstew-AS2 |
| Pstew-DC160 | Pstew-DC283 (IL, USA) |
| Pstew-ES9245 (IA, USA) | Pstew-GAL-E8 |
| Pstew-MU14110 | Pstew-Mu51 |
| Pstew-SS104 (IL, USA) | Pstew-SW1 (OH, USA) |
| Pstew-SW13 (IN, USA) | Pstew-SW14 (IN, USA) |
| Pstew-SW19 (KY, USA) | Pstew-SW2 (OH, USA) |
| Pstew-SW36 (TN, USA) | Pstew-SW39 (NC, USA) |
| Pstew-SW45 (MO, USA) | |
| Cross-reactions | |
|---|---|
| None Known | |
For a list of validated hosts, please see the Full Validation Report.
| Additional Documents | ||
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User Guide |
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| Option | Unit | Purchase |
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Pstew Reagent Set, 96 Testwells
SRA 52000/0096
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Shown in cart
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Pstew Reagent Set, 500 Testwells
SRA 52000/0500
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Shown in cart
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Pstew Reagent Set, 1000 Testwells
SRA 52000/1000
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Shown in cart
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Pstew Reagent Set, 5000 Testwells
SRA 52000/5000
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Shown in cart
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