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ELISA Test Kits for PMMoV (Pepper mild mottle virus)

This ELISA test is a qualitative serological assay for the detection of Pepper mild mottle virus (PMMoV) in ornamental leaves and vegetable leaves and seed. PMMoV is a member of the Tobamovirus genus known for their rod-shaped virus particles. It was able to consistently detect 1 PMMoV-infected seed in a subsample of 249 PMMoV-free pepper seeds.

If you would prefer to add a field-deployable assay to your testing toolkit, please check out Agdia's PMMoV ImmunoStrips®

Click here for other product documents such as the Reagent Set User Guide or PathoScreen Kit User Guide, click on the Documents tab for the full list.

See below for a summary of our ELISA format offerings. Not all formats are available for every test. See the Included Items tab for full details.

  • Reagent Set: Includes coating antibody, enzyme conjugate, and uncoated 96-well plate(s). Popular choice for established high-throughput laboratories.
  • PathoScreen Complete Kit: Includes pre-coated 96-well plate(s), enzyme conjugate, positive control, and all required buffers. Easiest format to get started with ELISA and requires the least amount of time and labor to run.
  • Capture Antibody: Only includes capture antibody. This is a partial component not usually ordered by itself. Unavailable on website. Call or email us to order.
  • Enzyme Conjugate: Includes enzyme conjugate and 96-well plate(s). This is a partial component not usually ordered by itself. Unavailable on website. Call or email us to order.

Reagent Set

Included in Reagent Set:

  • Capture antibody
  • Alkaline phosphatase enzyme conjugate
  • RUB6 Enzyme conjugate diluent, 1X Ready to use
  • 96-well microtiter plates, solid* (*strip in single plate set)
  • Reagent Set User Guide

Required but Not Included:

PathoScreen Kit (Format Not Available)


Coating Antibody

Included in Coating Antibody:

  • Capture antibody
  • 96-well microtiter plates, solid

Required but Not Included:

  • Only a partial component; see above for complete options

Enzyme Conjugate

Included in Enzyme Conjugate:

Required but Not Included:

  • Only a partial component; see above for complete options

For a full list of included items, including sizes, please see the Content List.

TWEEN® is a registered trademark of Croda International PLC.

Validation Report Summary

Test Label: Alkaline Phosphatase
Test Format: Double Antibody Sandwich (DAS) ELISA
Capture Reagent: Polyclonal
Detection Reagent: Polyclonal

Full Validation Report

Analytical Sensitivity
Limit of detection: 1:102,400 dilution of infected tissue (pathogen titer unknown)

Isolate Names / Geographic Strain Locations
PMMoV Canada Isolate PMMoV-CNU-1 (South Korea)3
PMMoV-GG1 (South Korea)1 PMMoV-Is (Israel)
PMMoV-J (Japan) PMMoV-JHD (South Korea)4
PMMoV-JI1 (South Korea)2 PMMoV Southern European Isolate

1PMMoV-GG1 has been externally reported to be detected.
2PMMoV-JI1 has been externally reported to be detected.
3PMMoV-CNU-1 has been externally reported to be detected.
4PMMoV-JHD has been externally reported to be detected.


Cross-reactions
African eggplant-associated virus (AEaV)2 Bell pepper mottle virus (BPeMV)1
Chili pepper mild mottle virus (CPMMoV) Odontoglossum ringspot virus (ORSV)1
Rehmannia mosaic virus (ReMV) Tobacco mild green mosaic virus (TMGMV)1
Tomato mosaic virus (ToMV)1

1Mild cross-reactivity was observed.
2African eggplant-associated virus (AEaV) has been reported to be a possible novel Tobamovirus.

For a list of validated hosts, please see the Full Validation Report.

Option Unit Purchase
PMMoV Reagent Set, 96 Testwells
SRA 37502/0096
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PMMoV Reagent Set, 500 Testwells
SRA 37502/0500
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PMMoV Reagent Set, 1000 Testwells
SRA 37502/1000
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PMMoV Reagent Set, 5000 Testwells
SRA 37502/5000
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