ELISA Reagent Set for Pepper mild mottle virus (PMMoV)

This ELISA test is a qualitative serological assay for the detection of Pepper mild mottle virus (PMMoV) in ornamental leaves and vegetable leaves and seed. PMMoV is a member of the Tobamovirus genus known for their rod-shaped virus particles. It was able to consistently detect 1 PMMoV-infected seed in a subsample of 249 PMMoV-free pepper seeds.

If you would prefer to add a field-deployable assay to your testing toolkit, please check out Agdia's PMMoV ImmunoStrips®

Click here for other product documents such as the User Guide or Buffer Formulations.

Included:

  • Capture antibody
  • Alkaline phosphatase enzyme conjugate
  • RUB6 Enzyme conjugate diluent, 1X Ready to use
  • 96-well microtiter plates, solid* (*strip in single plate set)
  • User Guide

Required but Not Included:

For a full list of included items, including sizes, please see the Content List.

TWEEN® is a registered trademark of Croda International PLC.

Validation Report Summary

Test Label: Alkaline Phosphatase
Test Format: Double Antibody Sandwich (DAS) ELISA
Capture Reagent: Polyclonal
Detection Reagent: Polyclonal

Full Validation Report


Analytical Sensitivity
Limit of detection: 1:102,400 dilution of infected tissue (pathogen titer unknown)

Isolate Names / Geographic Strain Locations
PMMoV Canadian Isolate PMMoV-Is (Israel)
PMMoV-J (Japan) PMMoV Southern European Isolate

Cross-reactions
Bell pepper mottle virus (BPeMV)1 Odontoglossum ringspot virus (ORSV)1
Tobacco mild green mosaic virus (TMGMV)1 Tomato mosaic virus (ToMV)1

1Mild cross-reactivity was observed.

For a list of validated hosts, please see the Full Validation Report.

Option Unit Purchase
Reagent Set for Pepper mild mottle virus (PMMoV), 96 testwells
SRA 37502/0096
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Reagent Set for Pepper mild mottle virus (PMMoV), 500 testwells
SRA 37502/0500
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Reagent Set for Pepper mild mottle virus (PMMoV), 1000 testwells
SRA 37502/1000
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Reagent Set for Pepper mild mottle virus (PMMoV), 5000 testwells
SRA 37502/5000
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