ELISA Test Kits for PMMoV (Pepper mild mottle virus)
This ELISA test is a qualitative serological assay for the detection of Pepper mild mottle virus (PMMoV) in ornamental leaves and vegetable leaves and seed. PMMoV is a member of the Tobamovirus genus known for their rod-shaped virus particles. It was able to consistently detect 1 PMMoV-infected seed in a subsample of 249 PMMoV-free pepper seeds.
If you would prefer to add a field-deployable assay to your testing toolkit, please check out Agdia's PMMoV ImmunoStrips®
Click here for other product documents such as the Reagent Set User Guide or PathoScreen Kit User Guide, click on the Documents tab for the full list.
See below for a summary of our ELISA format offerings. Not all formats are available for every test. See the Included Items tab for full details.
- Reagent Set: Includes coating antibody, enzyme conjugate, and uncoated 96-well plate(s). Popular choice for established high-throughput laboratories.
- PathoScreen Complete Kit: Includes pre-coated 96-well plate(s), enzyme conjugate, positive control, and all required buffers. Easiest format to get started with ELISA and requires the least amount of time and labor to run.
- Capture Antibody: Only includes capture antibody. This is a partial component not usually ordered by itself. Unavailable on website. Call or email us to order.
- Enzyme Conjugate: Includes enzyme conjugate and 96-well plate(s). This is a partial component not usually ordered by itself. Unavailable on website. Call or email us to order.
Reagent Set
Included in Reagent Set:
- Capture antibody
- Alkaline phosphatase enzyme conjugate
- RUB6 Enzyme conjugate diluent, 1X Ready to use
- 96-well microtiter plates, solid* (*strip in single plate set)
- Reagent Set User Guide
Required but Not Included:
- Buffer Pack (ACC 00333)
- Carbonate coating buffer, 10X concentrate
- PNP substrate buffer, 5X concentrate
- PNP substrate tablets, 5 mg each
- PBST buffer, powder
- General extract buffer
- TWEEN® 20
- Buffer Formulations from Chemicals
- Logsheet
PathoScreen Kit (Format Not Available)
Coating Antibody
Included in Coating Antibody:
- Capture antibody
- 96-well microtiter plates, solid
Required but Not Included:
- Only a partial component; see above for complete options
Enzyme Conjugate
Included in Enzyme Conjugate:
- Alkaline phosphatase enzyme conjugate
- Reagent Set User Guide
Required but Not Included:
- Only a partial component; see above for complete options
For a full list of included items, including sizes, please see the Content List.
TWEEN® is a registered trademark of Croda International PLC.
Validation Report Summary
Test Label: Alkaline Phosphatase |
Full Validation Report |
| Analytical Sensitivity |
|---|
| Limit of detection: 1:102,400 dilution of infected tissue (pathogen titer unknown) |
| Isolate Names / Geographic Strain Locations | |
|---|---|
| PMMoV Canada Isolate | PMMoV-CNU-1 (South Korea)3 |
| PMMoV-GG1 (South Korea)1 | PMMoV-Is (Israel) |
| PMMoV-J (Japan) | PMMoV-JHD (South Korea)4 |
| PMMoV-JI1 (South Korea)2 | PMMoV Southern European Isolate |
1PMMoV-GG1 has been externally reported to be detected.
2PMMoV-JI1 has been externally reported to be detected.
3PMMoV-CNU-1 has been externally reported to be detected.
4PMMoV-JHD has been externally reported to be detected.
| Cross-reactions | |
|---|---|
| Bell pepper mottle virus (BPeMV)1 | Chili pepper mild mottle virus (CPMMoV) |
| Odontoglossum ringspot virus (ORSV)1 | Tobacco mild green mosaic virus (TMGMV)1 |
| Tomato mosaic virus (ToMV)1 | |
1Mild cross-reactivity was observed.
For a list of validated hosts, please see the Full Validation Report.
| Additional Documents | ||
|---|---|---|
User Guide |
(Format Not Available) |
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| Option | Unit | Purchase |
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PMMoV Reagent Set, 96 Testwells
SRA 37502/0096
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Shown in cart
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PMMoV Reagent Set, 500 Testwells
SRA 37502/0500
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Shown in cart
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PMMoV Reagent Set, 1000 Testwells
SRA 37502/1000
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Shown in cart
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PMMoV Reagent Set, 5000 Testwells
SRA 37502/5000
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Shown in cart
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