ELISA Reagent Set for Prune dwarf virus (PDV)

This ELISA test is a qualitative serological assay for the detection of Prune dwarf virus (PDV) in stone fruit leaves. PDV is a member of the Ilarvirus genus known for their non-enveloped, quasi-spherical-shaped virus particles.

ELISA has proven to be an excellent diagnostic method for this pathogen as it reduces the time and labor involved with older techniques, such as grafting.

Click here for other product documents such as the User Guide or Buffer Formulations.

Included:

  • Capture antibody
  • Alkaline phosphatase enzyme conjugate
  • RUB6 enzyme conjugate diluent, 1X ready to use
  • General extract buffer 3, GEB3
  • TWEEN® 20
  • 96-well microtiter plates, solid* (*strip in single plate set)
  • User Guide

Required but Not Included:

For a full list of included items, including sizes, please see the Content List.

TWEEN® is a registered trademark of Croda International PLC.

Validation Report Summary

Test Label: Alkaline Phosphatase
Test Format: Double Antibody Sandwich (DAS) ELISA
Capture Reagent: Polyclonal
Detection Reagent: Polyclonal

Full Validation Report


Analytical Sensitivity
Limit of detection: 1:2,700 dilution of infected tissue (pathogen titer unknown)

Isolate Names / Geographic Strain Locations
This assay was designed to detect all strains and isolates of PDV. Thirty-five distinct samples of PDV have been experimentally proven to be detected, including PDV-876 (ATCC® PV-290™) (WI, USA).

Cross-reactions
None Known

For a list of validated hosts, please see the Full Validation Report.

Option Unit Purchase
Reagent Set for Prune dwarf virus (PDV), 96 testwells
SRA 98700/0096
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Reagent Set for Prune dwarf virus (PDV), 500 testwells
SRA 98700/0500
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Reagent Set for Prune dwarf virus (PDV), 1000 testwells
SRA 98700/1000
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Reagent Set for Prune dwarf virus (PDV), 5000 testwells
SRA 98700/5000
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