ELISA Reagent Set for Pectobacterium atrosepticum (Patro)
This ELISA test is a qualitative serological assay for the detection of Pectobacterium atrosepticum (Patro), the causal agent of potato blackleg disease and potato soft rot disease, in potato leaves, sprouts, and tubers. Patro was formally known as Erwinia carotovora subsp. atroseptica and Pectobacterium carotovora subsp. atroseptica.
This test system is based on monoclonal antibody clone 4F6, which was developed by Agriculture Canada and the Province of Alberta's Farming for the Future program.
Click here for other product documents such as the User Guide or Buffer Formulations.
Included:
- Capture antibody
- Detection antibody, bottle A
- Alkaline phosphatase enzyme conjugate, bottle B
- 96-well microtiter plates, solid* (*strip in single plate set)
- User Guide
Required but Not Included:
- Buffer Pack (ACC 00111)
- Carbonate coating buffer, 10X concentrate
- PNP substrate buffer, 5X concentrate
- PNP substrate tablets, 5 mg each
- PBST buffer, powder
- ECI buffer, 5X concentrate
- General extract buffer
- TWEEN® 20
- Buffer Formulations from Chemicals
- Logsheet
For a full list of included items, including sizes, please see the Content List.
TWEEN® is a registered trademark of Croda International PLC.
Validation Report Summary
Test Label: Alkaline Phosphatase |
Full Validation Report |
| Analytical Sensitivity |
|---|
| Limit of detection: 4.0x104 cells/mL |
| Isolate Names / Geographic Strain Locations | |
|---|---|
| Patro-196 (Serogroup XX) (Canada) (NCPPB# 3404) | Patro-E555 (Strain 198) (Serogroup XXII) (Canada) (NCPPB# 3406) |
| Patro-SR8 (Strain 31) (Serogroup I) (WI, USA) (ICPB# EA227) | |
| Isolate Names / Geographic Strain Locations Not Detected | |
| Patro-E17 (Strain 6) (Serogroup XVIII) (Canada) (ICPB# EA228) | |
| Cross-reactions | |
|---|---|
| Pectobacterium versatile (Strain F-139) (Netherlands) (ICPB# EC302) | |
For a list of validated hosts, please see the Full Validation Report.
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Reagent Set for Pectobacterium atrosepticum (Patro), 96 testwells
SRA 80003/0096
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Shown in cart
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Reagent Set for Pectobacterium atrosepticum (Patro), 500 testwells
SRA 80003/0500
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Shown in cart
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Reagent Set for Pectobacterium atrosepticum (Patro), 1000 testwells
SRA 80003/1000
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Shown in cart
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Reagent Set for Pectobacterium atrosepticum (Patro), 5000 testwells
SRA 80003/5000
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Shown in cart
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