ELISA Reagent Set for Pectobacterium atrosepticum (Patro)

This ELISA test is a qualitative serological assay for the detection of Pectobacterium atrosepticum (Patro), the causal agent of potato blackleg disease and potato soft rot disease, in potato leaves, sprouts, and tubers. Patro was formally known as Erwinia carotovora subsp. atroseptica and Pectobacterium carotovora subsp. atroseptica.

This test system is based on monoclonal antibody clone 4F6, which was developed by Agriculture Canada and the Province of Alberta's Farming for the Future program.

Click here for other product documents such as the User Guide or Buffer Formulations.

Included:

  • Capture antibody
  • Detection antibody, bottle A
  • Alkaline phosphatase enzyme conjugate, bottle B
  • 96-well microtiter plates, solid* (*strip in single plate set)
  • User Guide

Required but Not Included:

For a full list of included items, including sizes, please see the Content List.

TWEEN® is a registered trademark of Croda International PLC.

Validation Report Summary

Test Label: Alkaline Phosphatase
Test Format: Compound-ELISA
Capture Reagent: Polyclonal
Detection Reagent: Monoclonal

Full Validation Report


Analytical Sensitivity
Limit of detection: 4.0x104 cells/mL

Isolate Names / Geographic Strain Locations
Patro-196 (Serogroup XX) (Canada) (NCPPB# 3404) Patro-E555 (Strain 198) (Serogroup XXII) (Canada) (NCPPB# 3406)
Patro-SR8 (Strain 31) (Serogroup I) (WI, USA) (ICPB# EA227)
Isolate Names / Geographic Strain Locations Not Detected
Patro-E17 (Strain 6) (Serogroup XVIII) (Canada) (ICPB# EA228)

Cross-reactions
Pectobacterium versatile (Strain F-139) (Netherlands) (ICPB# EC302)

For a list of validated hosts, please see the Full Validation Report.

Option Unit Purchase
Reagent Set for Pectobacterium atrosepticum (Patro), 96 testwells
SRA 80003/0096
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Reagent Set for Pectobacterium atrosepticum (Patro), 500 testwells
SRA 80003/0500
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Reagent Set for Pectobacterium atrosepticum (Patro), 1000 testwells
SRA 80003/1000
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Reagent Set for Pectobacterium atrosepticum (Patro), 5000 testwells
SRA 80003/5000
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