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ELISA Test Kits for ORSV (Odontoglossum ringspot virus)

This ELISA test is a qualitative serological assay for the detection of Odontoglossum ringspot virus (ORSV) in orchid leaves. ORSV is a member of the Tobamovirus genus known for their rod-shaped virus particles.

ORSV is generally spread through orchid collections via contact with contaminated tools, because of this be sure to wash all sample cutting tools with a bleach solution before using them on another plant. This virus frequently causes severe symptoms in cultivated orchids, reducing the value of infected plants.

If you are testing orchid, you may also be interested in:

Agdia's ELISA for detection of Cymbidium mosaic virus (CymMV), another predominant pathogen in orchid cultivars.

Agdia's ImmunoStrip® for the detection of CymMV and ORSV: a simple and rapid testing device that can independently test for both pathogens. Perfect for use by hobbyists or greenhouse growers wishing to spot check their plants on-site.

Testing services orchid crop screen: send your samples directly to our laboratory to be tested against an entire panel of pathogens known to infect orchid.

Click here for other product documents such as the Reagent Set User Guide or PathoScreen Kit User Guide, click on the Documents tab for the full list.

See below for a summary of our ELISA format offerings. Not all formats are available for every test. See the Included Items tab for full details.

  • Reagent Set: Includes coating antibody, enzyme conjugate, and uncoated 96-well plate(s). Popular choice for established high-throughput laboratories.
  • PathoScreen Complete Kit: Includes pre-coated 96-well plate(s), enzyme conjugate, positive control, and all required buffers. Easiest format to get started with ELISA and requires the least amount of time and labor to run.
  • Capture Antibody: Only includes capture antibody. This is a partial component not usually ordered by itself. Unavailable on website. Call or email us to order.
  • Enzyme Conjugate: Includes enzyme conjugate and 96-well plate(s). This is a partial component not usually ordered by itself. Unavailable on website. Call or email us to order.

Reagent Set

Included in Reagent Set:

  • Capture antibody
  • Alkaline phosphatase enzyme conjugate
  • RUB6 Enzyme conjugate diluent, 1X Ready to use
  • 96-well microtiter plates, solid* (*strip in single plate set)
  • Reagent Set User Guide

Required but Not Included:

PathoScreen Kit (Format Not Available)


Coating Antibody

Included in Coating Antibody:

  • Capture antibody
  • 96-well microtiter plates, solid

Required but Not Included:

  • Only a partial component; see above for complete options

Enzyme Conjugate

Included in Enzyme Conjugate:

Required but Not Included:

  • Only a partial component; see above for complete options

For a full list of included items, including sizes, please see the Content List.

TWEEN® is a registered trademark of Croda International PLC.

Validation Report Summary

Test Label: Alkaline Phosphatase
Test Format: Double Antibody Sandwich (DAS) ELISA
Capture Reagent: Polyclonal
Detection Reagent: Polyclonal

Full Validation Report

Analytical Sensitivity
Limit of detection: 1:64,800 dilution of infected tissue (pathogen titer unknown)

Isolate Names / Geographic Strain Locations
This assay was designed to detect all strains and isolates of ORSV. Ten distinct samples of ORSV have been experimentally proven to be detected.

Cross-reactions
Virus Name Species Name
Kyuri green mottle mosaic virus (KGMMV) Tobamovirus kyuri

For a list of validated hosts, please see the Full Validation Report.

Option Unit Purchase
ORSV Reagent Set, 96 Testwells
SRA 54301/0096
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ORSV Reagent Set, 500 Testwells
SRA 54301/0500
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ORSV Reagent Set, 1000 Testwells
SRA 54301/1000
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ORSV Reagent Set, 5000 Testwells
SRA 54301/5000
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