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ELISA Reagent Set for Odontoglossum ringspot virus (ORSV)

This ELISA test is a qualitative serological assay for the detection of Odontoglossum ringspot virus (ORSV) in orchid leaves. ORSV is a member of the Tobamovirus genus known for their rod-shaped virus particles.

ORSV is generally spread through orchid collections via contact with contaminated tools, because of this be sure to wash all sample cutting tools with a bleach solution before using them on another plant. This virus frequently causes severe symptoms in cultivated orchids, reducing the value of infected plants.

Click here for other product documents such as the User Guide or Buffer Formulations.

If you are testing orchid, you may also be interested in:

Agdia's ELISA for detection of Cymbidium mosaic virus (CymMV), another predominant pathogen in orchid cultivars.

Agdia's ImmunoStrip® for the detection of CymMV and ORSV: a simple and rapid testing device that can independently test for both pathogens. Perfect for use by hobbyists or greenhouse growers wishing to spot check their plants on-site.

Testing services orchid crop screen: send your samples directly to our laboratory to be tested against an entire panel of pathogens known to infect orchid.


  • Capture antibody
  • Alkaline phosphatase enzyme conjugate
  • RUB6 Enzyme conjugate diluent, 1X Ready to use
  • 96-well microtiter plates, solid* (*strip in single plate set)
  • User Guide

Required but Not Included:

For a full list of included items, including sizes, please see the Content List.

TWEEN® is a registered trademark of Croda International PLC.

Validation Report Summary

Test Label: Alkaline Phosphatase
Test Format: Double Antibody Sandwich (DAS) ELISA
Capture Reagent: Polyclonal
Detection Reagent: Polyclonal

Full Validation Report

Analytical Sensitivity
Limit of detection: 1:64,800 dilution of infected tissue (pathogen titer unknown)

Isolate Names / Geographic Strain Locations
This assay was designed to detect all strains and isolates of ORSV. Ten distinct samples of ORSV have been experimentally proven to be detected.

Kyuri green mottle mosaic virus (KGMMV)

For a list of validated hosts, please see the Full Validation Report.

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Reagent Set for Odontoglossum ringspot virus (ORSV), 96 testwells
SRA 54301/0096
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Reagent Set for Odontoglossum ringspot virus (ORSV), 500 testwells
SRA 54301/0500
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Reagent Set for Odontoglossum ringspot virus (ORSV), 1000 testwells
SRA 54301/1000
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Reagent Set for Odontoglossum ringspot virus (ORSV), 5000 testwells
SRA 54301/5000
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