ELISA Reagent Set for Nemesia ring necrosis virus (NeRNV)

This ELISA test is a qualitative serological assay for the detection of Nemesia ring necrosis virus (NeRNV) in ornamental species. NeRNV is a member of the Tymovirus genus known for their non-enveloped, icosahedral, and isometric virus particles. Nemesia ring necrosis virus is known to infect multiple genera within Scrophulariaceae and Verbenaceae, including Alonsoa, Diascia, Nemesia and Verbena.

This test will detect all known isolates of NeRNV, and no cross-reactivity was observed with an extensive panel of common viruses of ornamental plants. Moreover, no host reaction was observed when tested against healthy plant samples representing 485 species/varieties from 91 genera. This test does cross-react with Scrophularia mottle virus (ScrMV).

Click here for other product documents such as the User Guide or Buffer Formulations.

Included:

  • Capture antibody
  • Alkaline phosphatase enzyme conjugate
  • MRS component, 5X concentrate
  • General extract buffer 3, GEB3
  • TWEEN® 20
  • 96-well microtiter plates, solid* (*strip in single plate set)
  • User Guide

Required but Not Included:

For a full list of included items, including sizes, please see the Content List.

TWEEN® is a registered trademark of Croda International PLC.

Validation Report Summary

Test Label: Alkaline Phosphatase
Test Format: Double Antibody Sandwich (DAS) ELISA
Capture Reagent: Polyclonal
Detection Reagent: Polyclonal

Full Validation Report


Analytical Sensitivity
Limit of detection: 1:648,000 dilution of infected tissue (pathogen titer unknown)

Isolate Names / Geographic Strain Locations
This assay was designed to detect all strains and isolates of NeRNV. One hundred sixty distinct samples of NeRNV, representing 146 different hosts, have been experimentally proven to be detected including samples of PV-0630 and PV-0631 from Germany and Israel, respectively.

Cross-reactions
Scrophularia mottle virus (ScrMV)

For a list of validated hosts, please see the Full Validation Report.

Option Unit Purchase
Reagent Set for Nemesia ring necrosis virus (NeRNV), 96 testwells
SRA 24300/0096
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Reagent Set for Nemesia ring necrosis virus (NeRNV), 500 testwells
SRA 24300/0500
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Reagent Set for Nemesia ring necrosis virus (NeRNV), 1000 testwells
SRA 24300/1000
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Reagent Set for Nemesia ring necrosis virus (NeRNV), 5000 testwells
SRA 24300/5000
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