ELISA Reagent Set for Melon necrotic spot virus (MNSV)
Tech Note: Alternate Seed Protocol
This ELISA test is a qualitative serological assay for the detection of Melon necrotic spot virus (MNSV) in cucurbit leaf and seed. MNSV is a member of the Gammacarmovirus genus known for their non-enveloped, spherical, icosahedral-shaped virus particles.
If you would prefer to add a field-deployable assay to your testing toolkit, please check out Agdia's MNSV ImmunoStrips®
Click here for other product documents such as the User Guide or Buffer Formulations.
Included:
- Capture antibody
- Alkaline phosphatase enzyme conjugate
- 96-well microtiter plates, solid* (*strip in single plate set)
- User Guide
- Tech Note
Required but Not Included:
- Buffer Pack (ACC 00111)
- Carbonate coating buffer, 10X concentrate
- PNP substrate buffer, 5X concentrate
- PNP substrate tablets, 5 mg each
- PBST buffer, powder
- ECI buffer, 5X concentrate
- General extract buffer
- TWEEN® 20
- Buffer Formulations from Chemicals
- Logsheet
For a full list of included items, including sizes, please see the Content List.
TWEEN® is a registered trademark of Croda International PLC.
Validation Report Summary
Test Label: Alkaline Phosphatase |
Full Validation Report |
| Analytical Sensitivity |
|---|
| Limit of detection: 1:38,800 dilution of infected tissue (pathogen titer unknown) |
| Isolate Names / Geographic Strain Locations | |
|---|---|
| MNSV-Al (Spain) | MNSV-Chiba (Japan) |
| MNSV-Kochi (Japan) | MNSV MAFF 260030 (Japan) |
| MNSV-NH (Japan) | MNSV-NK (Japan) |
| MNSV PV-0300 (Netherlands) | MNSV PV-0378 (Germany) |
| MNSV-Yamaguchi (Japan) | |
| Cross-reactions | |
|---|---|
| None Known | |
For a list of validated hosts, please see the Full Validation Report.
| Additional Documents | ||
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| Option | Unit | Purchase |
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Reagent Set for Melon necrotic spot virus (MNSV), 96 testwells
SRA 12402/0096
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Shown in cart
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Reagent Set for Melon necrotic spot virus (MNSV), 500 testwells
SRA 12402/0500
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Shown in cart
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Reagent Set for Melon necrotic spot virus (MNSV), 1000 testwells
SRA 12402/1000
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Shown in cart
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Reagent Set for Melon necrotic spot virus (MNSV), 5000 testwells
SRA 12402/5000
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Shown in cart
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