ELISA Test Kits for IYSV (Iris yellow spot virus)
This ELISA test is a qualitative serological assay for the detection of Iris yellow spot virus (IYSV), also known as Iris yellow spot orthotospovirus in ornamental and vegetable leaves. IYSV is a member of the Orthotospovirus genus known for their enveloped, spherical-shaped virus particles.
If you would prefer to add a field-deployable assay to your testing toolkit, please check out Agdia's IYSV ImmunoStrips®
Click here for other product documents such as the Reagent Set User Guide or PathoScreen Kit User Guide, click on the Documents tab for the full list.
See below for a summary of our ELISA format offerings. Not all formats are available for every test. See the Included Items tab for full details.
- Reagent Set: Includes coating antibody, enzyme conjugate, and uncoated 96-well plate(s). Popular choice for established high-throughput laboratories.
- PathoScreen Complete Kit: Includes pre-coated 96-well plate(s), enzyme conjugate, positive control, and all required buffers. Easiest format to get started with ELISA and requires the least amount of time and labor to run.
- Capture Antibody: Only includes capture antibody. This is a partial component not usually ordered by itself. Unavailable on website. Call or email us to order.
- Enzyme Conjugate: Includes enzyme conjugate and 96-well plate(s). This is a partial component not usually ordered by itself. Unavailable on website. Call or email us to order.
Reagent Set
Included in Reagent Set:
- Capture antibody
- Alkaline phosphatase enzyme conjugate
- 96-well microtiter plates, solid* (*strip in single plate set)
- Reagent Set User Guide
Required but Not Included:
- Buffer Pack (ACC 00111)
- Carbonate coating buffer, 10X concentrate
- PNP substrate buffer, 5X concentrate
- PNP substrate tablets, 5 mg each
- PBST buffer, powder
- ECI buffer, 5X concentrate
- General extract buffer
- TWEEN® 20
- Buffer Formulations from Chemicals
- Logsheet
PathoScreen Kit (Format Not Available)
Coating Antibody
Included in Coating Antibody:
- Capture antibody
- 96-well microtiter plates, solid
Required but Not Included:
- Only a partial component; see above for complete options
Enzyme Conjugate
Included in Enzyme Conjugate:
- Alkaline phosphatase enzyme conjugate
- Reagent Set User Guide
Required but Not Included:
- Only a partial component; see above for complete options
For a full list of included items, including sizes, please see the Content List.
TWEEN® is a registered trademark of Croda International PLC.
Validation Report Summary
|
Test Label: Alkaline Phosphatase |
Full Validation Report |
| Analytical Sensitivity |
|---|
| Limit of detection: 1:2,160 dilution of infected tissue (pathogen titer unknown) |
| Isolate Names / Geographic Strain Locations | |
|---|---|
| IYSV-3447 (Australia)1 | IYSV-5739 (Australia)1 |
| IYSV-5740 (Australia)1 | IYSV Israel isolate |
| IYSV Netherlands isolate | |
1IYSV-3447, IYSV-5739, and IYSV-5740 have been externally reported to be detected.
| Cross-reactions | |
|---|---|
| None Known | |
For a list of validated hosts, please see the Full Validation Report.
| Additional Documents | ||
|---|---|---|
User Guide |
|
|
|
|
|
|
|
|
|
|
|
|
||
| Option | Unit | Purchase |
|---|---|---|
|
IYSV Reagent Set, 96 Testwells
SRA 60500/0096
|
Shown in cart
|
|
|
IYSV Reagent Set, 500 Testwells
SRA 60500/0500
|
Shown in cart
|
|
|
IYSV Reagent Set, 1000 Testwells
SRA 60500/1000
|
Shown in cart
|
|
|
IYSV Reagent Set, 5000 Testwells
SRA 60500/5000
|
Shown in cart
|