ELISA Test Kits for HCRSV (Hibiscus chlorotic ringspot virus)
This ELISA test is a qualitative serological assay for the detection of Hibiscus chlorotic ringspot virus (HCRSV) in hibiscus leaves. HCRSV is a member of the Betacarmovirus genus known for their non-enveloped, spherical, icosahedral-shaped virus particles.
Click here for other product documents such as the Reagent Set User Guide or PathoScreen Kit User Guide, click on the Documents tab for the full list.
See below for a summary of our ELISA format offerings. Not all formats are available for every test. See the Included Items tab for full details.
- Reagent Set: Includes coating antibody, enzyme conjugate, and uncoated 96-well plate(s). Popular choice for established high-throughput laboratories.
- PathoScreen Complete Kit: Includes pre-coated 96-well plate(s), enzyme conjugate, positive control, and all required buffers. Easiest format to get started with ELISA and requires the least amount of time and labor to run.
- Capture Antibody: Only includes capture antibody. This is a partial component not usually ordered by itself. Unavailable on website. Call or email us to order.
- Enzyme Conjugate: Includes enzyme conjugate and 96-well plate(s). This is a partial component not usually ordered by itself. Unavailable on website. Call or email us to order.
Reagent Set
Included in Reagent Set:
- Capture antibody
- Alkaline phosphatase enzyme conjugate
- 96-well microtiter plates, solid* (*strip in single plate set)
- Reagent Set User Guide
Required but Not Included:
- Buffer Pack (ACC 00111)
- Carbonate coating buffer, 10X concentrate
- PNP substrate buffer, 5X concentrate
- PNP substrate tablets, 5 mg each
- PBST buffer, powder
- ECI buffer, 5X concentrate
- General extract buffer
- TWEEN® 20
- Buffer Formulations from Chemicals
- Logsheet
PathoScreen Kit (Format Not Available)
Coating Antibody
Included in Coating Antibody:
- Capture antibody
- 96-well microtiter plates, solid
Required but Not Included:
- Only a partial component; see above for complete options
Enzyme Conjugate
Included in Enzyme Conjugate:
- Alkaline phosphatase enzyme conjugate
- Reagent Set User Guide
Required but Not Included:
- Only a partial component; see above for complete options
For a full list of included items, including sizes, please see the Content List.
TWEEN® is a registered trademark of Croda International PLC.
Validation Report Summary
Test Label: Alkaline Phosphatase |
Full Validation Report |
| Analytical Sensitivity |
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| Limit of detection: 1:12,960 dilution of infected tissue (pathogen titer unknown) |
| Isolate Names / Geographic Strain Locations | |
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| This assay was designed to detect all strains and isolates of HCRSV. Ten distinct samples of HCRSV have been experimentally proven to be detected including HCRSV-Ita-1 (Italy). | |
| Cross-reactions | |
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| None Known | |
For a list of validated hosts, please see the Full Validation Report.
| Additional Documents | ||
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User Guide |
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| Option | Unit | Purchase |
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HCRSV Reagent Set, 96 Testwells
SRA 12300/0096
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Shown in cart
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HCRSV Reagent Set, 500 Testwells
SRA 12300/0500
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Shown in cart
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HCRSV Reagent Set, 1000 Testwells
SRA 12300/1000
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Shown in cart
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HCRSV Reagent Set, 5000 Testwells
SRA 12300/5000
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Shown in cart
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