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ELISA Reagent Set for Groundnut ringspot virus & Tomato chlorotic spot virus (GRSV & TCSV)

Tech Note: Product Improvement

This ELISA test is a qualitative serological assay for the detection of Groundnut ringspot virus & Tomato chlorotic spot virus (GRSV & TCSV) in ornamental and vegetable leaves. GRSV & TCSV is a member of the Orthotospovirus genus known for their enveloped, spherical-shaped virus particles.

Click here for other product documents such as the User Guide or Buffer Formulations.

Included:

  • Capture antibody
  • Alkaline phosphatase enzyme conjugate
  • 96-well microtiter plates, solid* (*strip in single plate set)
  • User Guide
  • Tech Note

Required but Not Included:

For a full list of included items, including sizes, please see the Content List.

TWEEN® is a registered trademark of Croda International PLC.

Validation Report Summary

Test Label: Alkaline Phosphatase
Test Format: Double Antibody Sandwich (DAS) ELISA
Capture Reagent: Polyclonal
Detection Reagent: Polyclonal

Full Validation Report


Analytical Sensitivity
Limit of detection: 1:10,000 dilution of infected tissue (pathogen titer unknown)

Isolate Names / Geographic Strain Locations
This assay was designed to detect all strains and isolates of GRSV & TCSV. Thirty-five distinct samples of GRSV & TCSV have been experimentally proven to be detected.

Cross-reactions
Virus Name Species Name
Groundnut bud necrosis virus (GBNV) Orthotospovirus arachinecrosis
Tomato spotted wilt virus (TSWV) Orthotospovirus tomatomaculae
Watermelon silver mottle virus (WSMoV) Orthotospovirus citrullomaculosi

For a list of validated hosts, please see the Full Validation Report.

Option Unit Purchase
Reagent Set for Groundnut ringspot virus & Tomato chlorotic spot virus (GRSV & TCSV), 96 testwells
SRA 61000/0096
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Reagent Set for Groundnut ringspot virus & Tomato chlorotic spot virus (GRSV & TCSV), 500 testwells
SRA 61000/0500
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Reagent Set for Groundnut ringspot virus & Tomato chlorotic spot virus (GRSV & TCSV), 1000 testwells
SRA 61000/1000
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Reagent Set for Groundnut ringspot virus & Tomato chlorotic spot virus (GRSV & TCSV), 5000 testwells
SRA 61000/5000
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