ELISA Reagent Set for Erwinia amylovora (Ea)
This ELISA test is a qualitative serological assay for the detection of Erwinia amylovora (Ea), the causal agent of fire blight, in apple and pear leaves, fruit, and stems. Ea is a member of the Erwinia genus known for their primarily rod-shaped Gram-negative bacterial particles. This ELISA also detects Erwinia pyrifoliae, a closely related species known to cause tissue necrosis on pear in Asia and strawberries in Europe.
If you would prefer to add a field-deployable assay to your testing toolkit, please check out Agdia's Ea ImmunoStrips®
Click here for other product documents such as the User Guide or Buffer Formulations.
Included:
- Capture antibody
- Detection antibody, bottle A
- Alkaline phosphatase enzyme conjugate, bottle B
- 96-well microtiter plates, solid* (*strip in single plate set)
- User Guide
Required but Not Included:
- Buffer Pack (ACC 00111)
- Carbonate coating buffer, 10X concentrate
- PNP substrate buffer, 5X concentrate
- PNP substrate tablets, 5 mg each
- PBST buffer, powder
- ECI buffer, 5X concentrate
- General extract buffer
- TWEEN® 20
- Buffer Formulations from Chemicals
- Logsheet
For a full list of included items, including sizes, please see the Content List.
TWEEN® is a registered trademark of Croda International PLC.
Validation Report Summary
Test Label: Alkaline Phosphatase |
Full Validation Report |
| Analytical Sensitivity |
|---|
| Limit of detection: 4x105 to 4x106 cells/mL |
| Isolate Names / Geographic Strain Locations | |
|---|---|
| Ea-CA11 (MI, USA) | Ea-CA3R (CA, USA) |
| Ea-CFBP1430 (France) | Ea-CTBT1-1 (CT, USA) |
| Ea-CTMF03-1 (CT, USA) | Ea-CTST01-1 (CT, USA) |
| Ea-Ea110 (MI, USA) | Ea-Ea322 (France) |
| Ea-Ea7-96 (Canada) | Ea-EJP557 (Japan) |
| Ea-IH3-1 (SC, USA) | Ea-LA092 (WA, USA) |
| Ea-LebB66 (Lebanon) | Ea-MAGFLF 2 (MA, USA) |
| Ea-MASHBO02-2 | Ea-MR1 (USA) |
| Ea-NHSB01-1 (NH, USA) | Ea-nydr001 |
| Ea-OR1 (OR, USA) | Ea-OR6 (OR, USA) |
| Ea-RBA4 (MI, USA) | Ea-RISTBO01-2 (RI, USA) |
| Ea-UT5P4 (UT, USA) | Ea-UTRJ2 (UT, USA) |
| Ea-VTDMSF02 (VT, USA) | Ea-WSDA87-73 (WA, USA) |
| Cross-reactions | |
|---|---|
| Erwinia pyrifoliae | |
For a list of validated hosts, please see the Full Validation Report.
| Additional Documents | ||
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Reagent Set for Erwinia amylovora (Ea), 96 testwells
SRA 52101/0096
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Shown in cart
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Reagent Set for Erwinia amylovora (Ea), 500 testwells
SRA 52101/0500
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Shown in cart
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Reagent Set for Erwinia amylovora (Ea), 1000 testwells
SRA 52101/1000
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Shown in cart
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Reagent Set for Erwinia amylovora (Ea), 5000 testwells
SRA 52101/5000
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Shown in cart
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