ELISA Reagent Set for Citrus tristeza virus (CTV)
This ELISA test is a qualitative serological assay for the detection of Citrus tristeza virus (CTV) in leaf and stem of citrus producing plant species. CTV is a member of the Closterovirus genus known for their Non-enveloped, flexuous and exceptionally long, filamentous virus particles. CTV is a phloem limited pathogen and it is important that adequate sample extraction is performed prior to testing. Samples should be taken from young green shoots for best results.
If you would prefer to add a field-deployable assay to your testing toolkit, please check out Agdia's CTV ImmunoStrips®
Click here for other product documents such as the User Guide or Buffer Formulations.
Included:
- Capture antibody
- Detection antibody, bottle A
- Alkaline phosphatase enzyme conjugate, bottle B
- 96-well microtiter plates, solid* (*strip in single plate set)
- User Guide
Required but Not Included:
- Buffer Pack (ACC 00111)
- Carbonate coating buffer, 10X concentrate
- PNP substrate buffer, 5X concentrate
- PNP substrate tablets, 5 mg each
- PBST buffer, powder
- ECI buffer, 5X concentrate
- General extract buffer
- TWEEN® 20
- Buffer Formulations from Chemicals
- Logsheet
For a full list of included items, including sizes, please see the Content List.
TWEEN® is a registered trademark of Croda International PLC.
Validation Report Summary
Test Label: Alkaline Phosphatase |
Full Validation Report |
Analytical Sensitivity |
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Limit of detection: 1:3,600 dilution of infected tissue (pathogen titer unknown) |
Isolate Names / Geographic Strain Locations | |
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CTV-B7 (South Africa) | CTV-B12 (Brazil) |
CTV-B13 (Brazil) | CTV-B14 (Brazil) |
CTV-B17 (Brazil) | CTV-B23 (Israel) |
CTV-B26 (Israel) | CTV-B29 (Japan) |
CTV-B30 (Japan) | CTV-B31 (Japan) |
CTV-B35 (Spain) | CTV-B37 (China/Taiwan) |
CTV-B59 (South Africa) | CTV-B65 (China) |
CTV-B77 (Brazil) | CTV-B140 |
CTV-B144 (China) | CTV-B151 (California) |
CTV-B186 (Japan) | CTV-B188 (Japan) |
CTV-B190 (Japan) | CTV-B192 (Corsica) |
CTV-B213 (South Korea) | CTV-B214 |
CTV-B310 (Cuba) | CTV-B334 (California) |
CTV-B352 (California) | CTV-FD284 B35 |
CTV-FD289 B77 | CTV-FD302 B12 |
CTV-FD304 B111 | CTV-FD308 B192 |
CTV-FD317 B31 | CTV-FD320 B31 |
CTV-FD321 B77 | CTV-FD331 B188 |
CTV-FD333 B67 | CTV-FD337 B65 |
CTV-FL216 | CTV-FL278 (Florida) |
CTV-FS674 (Florida) | CTV-T30 (mild) (Florida) |
CTV-T36 (severe) (Florida) | CTV-92 |
CTV-107 | CTV-150 (California) |
CTV-162 (California) | CTV-519 |
CTV-558 | CTV-93179 |
Cross-reactions | |
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None Known |
For a list of validated hosts, please see the Full Validation Report.
Additional Documents | ||
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Option | Unit | Purchase |
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Reagent Set for Citrus tristeza virus (CTV), 96 testwells
SRA 78900/0096
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Shown in cart
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Reagent Set for Citrus tristeza virus (CTV), 500 testwells
SRA 78900/0500
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Shown in cart
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Reagent Set for Citrus tristeza virus (CTV), 1000 testwells
SRA 78900/1000
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Shown in cart
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Reagent Set for Citrus tristeza virus (CTV), 5000 testwells
SRA 78900/5000
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Shown in cart
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