ELISA Reagent Set for Cucumber mosaic virus -subgroup II (CMV II)
Tech Note: Coating Antibody Information
This ELISA test is a qualitative serological assay for the detection of Cucumber mosaic virus - subgroup II (CMV II) in cucurbit leaves. CMV II is a member of the Cucumovirus genus known for their non-enveloped, spherical virus particles.
This test system is specific for CMV subgroup II (CMV-ToRS). It does not detect CMV subgroup I (CMV-DTL) or other cucumoviruses including Peanut stunt virus and Tomato aspermy virus. For more technical information click here.
If you would prefer to add a field-deployable assay to your testing toolkit, please check out Agdia's CMV ImmunoStrips® (Will detect both CMV I and CMV II).
Click here for other product documents such as the User Guide or Buffer Formulations.
Included:
- Capture antibody
- Detection antibody, bottle A
- Alkaline phosphatase enzyme conjugate, bottle B
- 96-well microtiter plates, solid* (*strip in single plate set)
- User Guide
- Tech Note
Required but Not Included:
- Buffer Pack (ACC 00111)
- Carbonate coating buffer, 10X concentrate
- PNP substrate buffer, 5X concentrate
- PNP substrate tablets, 5 mg each
- PBST buffer, powder
- ECI buffer, 5X concentrate
- General extract buffer
- TWEEN® 20
- Buffer Formulations from Chemicals
- Logsheet
For a full list of included items, including sizes, please see the Content List.
TWEEN® is a registered trademark of Croda International PLC.
Validation Report Summary
Test Label: Alkaline Phosphatase |
Full Validation Report |
| Analytical Sensitivity |
|---|
| Limit of detection: 1:90 dilution of infected tissue (pathogen titer unknown) |
| Isolate Names / Geographic Strain Locations | |
|---|---|
| CMV ATCC® PV-549™ | CMV-100 (New Zealand) |
| CMV-101 (New Zealand) | CMV-107 (New Zealand) |
| CMV-110 (New Zealand) | CMV-119 (New Zealand) |
| CMV-223 (New Zealand) | CMV-88 (New Zealand) |
| CMV-90 (New Zealand) | CMV-94 (New Zealand) |
| CMV-97 (New Zealand) | CMV-98 (New Zealand) |
| CMV-C105 (MI, USA) | CMV-C109 (CA, USA) |
| CMV-F354 seed transmissible (CA, USA) | CMV-Kaper S (ATCC® PV-242™) |
| CMV-LS (ATCC® PV-542™) (NY, USA) | CMV-LS (NY, USA) |
| CMV-Mal1 (USA) | CMV-Q (ATCC® PV-289™) (Australia) |
| CMV-Q (Australia) | CMV-R (France) |
| CMV-R76B (England) | CMV-Rm (France) |
| CMV-RS15 (Taiwan) | CMV-S (South Africa) |
| CMV-S2A (NY, USA) | CMV-S390 (TX, USA) |
| CMV-T1T2Q3 | CMV-U (Austraila) |
| CMV-Watercress | |
| Cross-reactions | |
|---|---|
| None Known | |
For a list of validated hosts, please see the Full Validation Report.
| Additional Documents | ||
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| Option | Unit | Purchase |
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Reagent Set for Cucumber mosaic virus subgroup II (CMV II), 96 testwells
SRA 44800/0096
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Shown in cart
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Reagent Set for Cucumber mosaic virus subgroup II (CMV II), 500 testwells
SRA 44800/0500
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Shown in cart
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Reagent Set for Cucumber mosaic virus subgroup II (CMV II), 1000 testwells
SRA 44800/1000
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Shown in cart
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Reagent Set for Cucumber mosaic virus subgroup II (CMV II), 5000 testwells
SRA 44800/5000
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Shown in cart
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