ELISA Reagent Set for Clavibacter michiganensis subsp. tessellarius (Cmt)
This ELISA test is a qualitative serological assay for the detection of Clavibacter michiganensis subsp. tessellarius (Cmt), the causal agent of bacterial mosaic, in wheat leaf and seed. Cmt is a member of the Clavibacter genus known for their aerobic, non-motile, Gram-positive, non-sporing, curved rod shaped bacteria.
Click here for other product documents such as the User Guide or Buffer Formulations.
Included:
- Capture antibody
- Alkaline phosphatase enzyme conjugate
- 96-well microtiter plates, solid* (*strip in single plate set)
- User Guide
Required but Not Included:
- Buffer Pack (ACC 00111)
- Carbonate coating buffer, 10X concentrate
- PNP substrate buffer, 5X concentrate
- PNP substrate tablets, 5 mg each
- PBST buffer, powder
- ECI buffer, 5X concentrate
- General extract buffer
- TWEEN® 20
- Buffer Formulations from Chemicals
- Logsheet
For a full list of included items, including sizes, please see the Content List.
TWEEN® is a registered trademark of Croda International PLC.
Validation Report Summary
Test Label: Alkaline Phosphatase |
Full Validation Report |
| Analytical Sensitivity |
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| Limit of detection: 7.0x107 CFU/mL |
| Isolate Names / Geographic Strain Locations | |
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| This assay was designed to detect all strains and isolates of Cmt. Thirteen distinct samples of Cmt have been experimentally proven to be detected including samples of strain 78181. | |
| Cross-reactions | |
|---|---|
| Clavibacter flaccumfaciens subsp. poinsettiae | Clavibacter michiganensis pv. insidiosus (Cmi) |
| Clavibacter michiganensis pv. nebraskensis (Cmn) | Clavibacter michiganensis pv. sepedonicus (Cms) |
| Clavibacter poinsettiae | |
For a list of validated hosts, please see the Full Validation Report.
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Reagent Set for Clavibacter michiganensis subsp. tessellarius (Cmt), 96 testwells
SRA 44100/0096
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Shown in cart
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Reagent Set for Clavibacter michiganensis subsp. tessellarius (Cmt), 500 testwells
SRA 44100/0500
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Shown in cart
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Reagent Set for Clavibacter michiganensis subsp. tessellarius (Cmt), 1000 testwells
SRA 44100/1000
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Shown in cart
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Reagent Set for Clavibacter michiganensis subsp. tessellarius (Cmt), 5000 testwells
SRA 44100/5000
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Shown in cart
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