ELISA Test Kits for Cms (Clavibacter michiganensis subsp. sepedonicus)
This ELISA test is a qualitative serological assay for the detection of Clavibacter michiganensis subsp. sepedonicus (Cms), the causal agent of Bacterial ring rot (BRR), in Potato leaves and tubers. Composite samples of 200 tuber cores can be used and still achieve reliable results. Cms is a member of the Clavibacter genus known for their aerobic, non-motile, Gram-positive, non-sporing, curved rod shaped bacteria.
Click here for other product documents such as the Reagent Set User Guide or PathoScreen Kit User Guide, click on the Documents tab for the full list.
See below for a summary of our ELISA format offerings. Not all formats are available for every test. See the Included Items tab for full details.
- Reagent Set: Includes coating antibody, enzyme conjugate, and uncoated 96-well plate(s). Popular choice for established high-throughput laboratories.
- PathoScreen Complete Kit: Includes pre-coated 96-well plate(s), enzyme conjugate, positive control, and all required buffers. Easiest format to get started with ELISA and requires the least amount of time and labor to run.
- Capture Antibody: Only includes capture antibody. This is a partial component not usually ordered by itself. Unavailable on website. Call or email us to order.
- Enzyme Conjugate: Includes enzyme conjugate and 96-well plate(s). This is a partial component not usually ordered by itself. Unavailable on website. Call or email us to order.
Reagent Set (Format Not Available)
PathoScreen Kit
Included in PathoScreen Kit:
- Antibody-coated 96-well microtiter plates
- Alkaline phosphatase enzyme conjugate
- Nonfat Dried Milk
- PNP substrate tablets, 5 mg each
- PNP substrate buffer, 5X concentrate
- Positive control
- PBST buffer, 20X concentrate, 50 mL
- TWEEN® 20
- PathoScreen User Guide
Required but Not Included:
Coating Antibody (Format Not Available)
Enzyme Conjugate
Included in Enzyme Conjugate:
- Alkaline phosphatase enzyme conjugate
- PathoScreen User Guide
Required but Not Included:
- Only a partial component; see above for complete options
For a full list of included items, including sizes, please see the Content List.
TWEEN® is a registered trademark of Croda International PLC.
Validation Report Summary
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Test Label: Alkaline Phosphatase |
Full Validation Report |
| Analytical Sensitivity |
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| Limit of detection: 2.0 x 105 cells/mL |
| Isolate Names / Geographic Strain Locations | |
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| This assay was designed to detect all strains and isolates of Cms. Forty-seven distinct samples of Cms have been experimentally proven to be detected, including samples of mucoid and non-mucoid strains. | |
| Cross-reactions | |
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| None Known | |
For a list of validated hosts, please see the Full Validation Report.
| Additional Documents | ||
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User Guide (Format Not Available) |
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| Option | Unit | Purchase |
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Cms PathoScreen® Complete Kit, 96 Testwells
PSA 70002/0096
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Shown in cart
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Cms PathoScreen® Complete Kit, 288 Testwells
PSA 70002/0288
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Shown in cart
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Cms PathoScreen® Complete Kit, 480 Testwells
PSA 70002/0480
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Shown in cart
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