ELISA Test Kits for BWYV (Beet western yellows virus)
Tech Note: Protocol Change and Product Improvement
This ELISA test is a qualitative serological assay for the detection of Beet western yellows virus (BWYV) in ornamental and vegetable leaves. BWYV is a member of the Polerovirus genus known for their Non-enveloped, spherical-shaped virus particles.
Click here for other product documents such as the Reagent Set User Guide or PathoScreen Kit User Guide, click on the Documents tab for the full list.
See below for a summary of our ELISA format offerings. Not all formats are available for every test. See the Included Items tab for full details.
- Reagent Set: Includes coating antibody, enzyme conjugate, and uncoated 96-well plate(s). Popular choice for established high-throughput laboratories.
- PathoScreen Complete Kit: Includes pre-coated 96-well plate(s), enzyme conjugate, positive control, and all required buffers. Easiest format to get started with ELISA and requires the least amount of time and labor to run.
- Capture Antibody: Only includes capture antibody. This is a partial component not usually ordered by itself. Unavailable on website. Call or email us to order.
- Enzyme Conjugate: Includes enzyme conjugate and 96-well plate(s). This is a partial component not usually ordered by itself. Unavailable on website. Call or email us to order.
Reagent Set
Included in Reagent Set:
- Capture antibody
- Detection antibody, bottle A
- Alkaline phosphatase enzyme conjugate, bottle B
- 96-well microtiter plates, solid* (*strip in single plate set)
- Reagent Set User Guide
- Tech Note
Required but Not Included:
- Buffer Pack (ACC 00111)
- Carbonate coating buffer, 10X concentrate
- PNP substrate buffer, 5X concentrate
- PNP substrate tablets, 5 mg each
- PBST buffer, powder
- ECI buffer, 5X concentrate
- General extract buffer
- TWEEN® 20
- Buffer Formulations from Chemicals
- Logsheet
PathoScreen Kit
Included in PathoScreen Kit:
- Antibody-coated 96-well microtiter plates
- Detection antibody, bottle A
- Alkaline phosphatase enzyme conjugate, bottle B
- ECI buffer, 5X concentrate
- PNP substrate tablets, 5 mg each
- PNP substrate buffer, 5X concentrate
- Positive control
- PBST buffer, 20X concentrate, 50 mL
- TWEEN® 20
- General extract buffer, GEB
- PathoScreen User Guide
- Tech Note
Required but Not Included:
Coating Antibody
Included in Coating Antibody:
- Capture antibody
- 96-well microtiter plates, solid
Required but Not Included:
- Only a partial component; see above for complete options
Enzyme Conjugate
Included in Enzyme Conjugate:
- Detection antibody, bottle A
- Alkaline phosphatase enzyme conjugate, bottle B
- Reagent Set User Guide
- Tech Note
Required but Not Included:
- Only a partial component; see above for complete options
For a full list of included items, including sizes, please see the Content List.
TWEEN® is a registered trademark of Croda International PLC.
Validation Report Summary
Test Label: Alkaline Phosphatase |
Full Validation Report |
| Analytical Sensitivity |
|---|
| Limit of detection: 1:5,000 dilution of infected tissue (pathogen titer unknown) |
| Isolate Names / Geographic Strain Locations | |
|---|---|
| This assay was designed to detect all strains and isolates of BWYV. Eighty distinct samples of BWYV have been experimentally proven to be detected. |
| Cross-reactions | |
|---|---|
| Beet chlorosis virus (BChV) | Beet mild yellowing virus (BMYV) |
| Cereal yellow dwarf virus RPS (CYDV-RPS) | Cereal yellow dwarf virus RPV (CYDV-RPV) |
For a list of validated hosts, please see the Full Validation Report.
| Option | Unit | Purchase |
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Agdia Set BWYV alkphos
SRA 32100/0096
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Shown in cart
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Agdia Set BWYV alkphos
SRA 32100/0500
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Shown in cart
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Agdia Set BWYV alkphos
SRA 32100/1000
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Shown in cart
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Agdia Set BWYV alkphos
SRA 32100/5000
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Shown in cart
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BWYV PathoScreen® Complete Kit, 96 Testwells
PSA 32100/0096
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Shown in cart
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BWYV PathoScreen® Complete Kit, 288 Testwells
PSA 32100/0288
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Shown in cart
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BWYV PathoScreen® Complete Kit, 480 Testwells
PSA 32100/0480
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Shown in cart
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