Search Products
loading...

ELISA Test Kits for BWYV (Beet western yellows virus)

Tech Note: Protocol Change and Product Improvement

This ELISA test is a qualitative serological assay for the detection of Beet western yellows virus (BWYV) in ornamental and vegetable leaves. BWYV is a member of the Polerovirus genus known for their Non-enveloped, spherical-shaped virus particles.

Click here for other product documents such as the Reagent Set User Guide or PathoScreen Kit User Guide, click on the Documents tab for the full list.

See below for a summary of our ELISA format offerings. Not all formats are available for every test. See the Included Items tab for full details.

  • Reagent Set: Includes coating antibody, enzyme conjugate, and uncoated 96-well plate(s). Popular choice for established high-throughput laboratories.
  • PathoScreen Complete Kit: Includes pre-coated 96-well plate(s), enzyme conjugate, positive control, and all required buffers. Easiest format to get started with ELISA and requires the least amount of time and labor to run.
  • Capture Antibody: Only includes capture antibody. This is a partial component not usually ordered by itself. Unavailable on website. Call or email us to order.
  • Enzyme Conjugate: Includes enzyme conjugate and 96-well plate(s). This is a partial component not usually ordered by itself. Unavailable on website. Call or email us to order.

Reagent Set

Included in Reagent Set:

  • Capture antibody
  • Detection antibody, bottle A
  • Alkaline phosphatase enzyme conjugate, bottle B
  • 96-well microtiter plates, solid* (*strip in single plate set)
  • Reagent Set User Guide
  • Tech Note

Required but Not Included:

PathoScreen Kit

Included in PathoScreen Kit:

  • Antibody-coated 96-well microtiter plates
  • Detection antibody, bottle A
  • Alkaline phosphatase enzyme conjugate, bottle B
  • ECI buffer, 5X concentrate
  • PNP substrate tablets, 5 mg each
  • PNP substrate buffer, 5X concentrate
  • Positive control
  • PBST buffer, 20X concentrate, 50 mL
  • TWEEN® 20
  • General extract buffer, GEB
  • PathoScreen User Guide
  • Tech Note

Coating Antibody

Included in Coating Antibody:

  • Capture antibody
  • 96-well microtiter plates, solid

Required but Not Included:

  • Only a partial component; see above for complete options

Enzyme Conjugate

Included in Enzyme Conjugate:

Required but Not Included:

  • Only a partial component; see above for complete options

For a full list of included items, including sizes, please see the Content List.

TWEEN® is a registered trademark of Croda International PLC.

Validation Report Summary

Test Label: Alkaline Phosphatase
Test Format: Compound-ELISA
Capture Reagent: Polyclonal
Detection Reagent: Monoclonal

Full Validation Report


Analytical Sensitivity
Limit of detection: 1:5,000 dilution of infected tissue (pathogen titer unknown)

Isolate Names / Geographic Strain Locations
This assay was designed to detect all strains and isolates of BWYV. Eighty distinct samples of BWYV have been experimentally proven to be detected.

Cross-reactions
Beet chlorosis virus (BChV) Beet mild yellowing virus (BMYV)
Cereal yellow dwarf virus RPS (CYDV-RPS) Cereal yellow dwarf virus RPV (CYDV-RPV)

For a list of validated hosts, please see the Full Validation Report.

Option Unit Purchase
BWYV PathoScreen® Complete Kit, 96 Testwells
PSA 32100/0096
Shown in cart
Sign in to Buy In Stock
BWYV PathoScreen® Complete Kit, 288 Testwells
PSA 32100/0288
Shown in cart
Sign in to Buy In Stock
BWYV PathoScreen® Complete Kit, 480 Testwells
PSA 32100/0480
Shown in cart
Sign in to Buy In Stock