ELISA Reagent Set for Broad bean wilt virus 1,2 (BBWV-1,2)

This ELISA test is a qualitative serological assay for the detection of Broad bean wilt virus 1 and 2 (BBWV-1,2) in ornamental leaves. BBWV-1,2 is a member of the Fabavirus genus known for their non-enveloped, icosahedral-shaped virus particles.

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Included:

  • Capture antibody
  • Alkaline phosphatase enzyme conjugate
  • General extract buffer 3, GEB3
  • TWEEN® 20
  • 96-well microtiter plates, solid* (*strip in single plate set)
  • User Guide

Required but Not Included:

For a full list of included items, including sizes, please see the Content List.

TWEEN® is a registered trademark of Croda International PLC.

Validation Report Summary

Test Label: Alkaline Phosphatase
Test Format: Double Antibody Sandwich (DAS) ELISA
Capture Reagent: Polyclonal
Detection Reagent: Polyclonal

Full Validation Report


Analytical Sensitivity
Limit of detection: 1:51,200 dilution of infected tissue (pathogen titer unknown)

Isolate Names / Geographic Strain Locations
BBWV-1,2 ATCC® PV-131™ BBWV-1,2 ATCC® PV-132™
BBWV-1,2-Benthamiana BBWV-1,2-IFA 62
BBWV-1,2-IFA 168 BBWV-1,2-JV (Germany)
BBWV-1,2-K407b (Germany) BBWV-1,2-K442a (Germany)
BBWV-1,2-K497 (Germany) BBWV-1,2-K1926 (Germany)
BBWV-1,2-K1927 (Germany) BBWV-1,2-K2194 (Germany)
BBWV-1,2-K2505 (Germany) BBWV-1,2-K2588 (Germany)
BBWV-1,2-K2983 (Germany) BBWV-1,2-Lynchnis
BBWV-1,2 PV-0221 (Italy) BBWV-1,2 PV-0537 (Spinach)
BBWV-1,2 PV-0548 (Syria) BBWV-1,2 PV-0549 (Germany)
BBWV-1,2 PV-0550 (Germany) BBWV-1,2 PV-0862 (Germany)
BBWV-1,2 PV-0881 (Austria)

Cross-reactions
None Known

For a list of validated hosts, please see the Full Validation Report.

Option Unit Purchase
Reagent Set for Broad bean wilt virus 1 and 2 (BBWV 1,2), 96 testwells
SRA 46202/0096
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Reagent Set for Broad bean wilt virus 1 and 2 (BBWV 1,2), 500 testwells
SRA 46202/0500
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Reagent Set for Broad bean wilt virus 1 and 2 (BBWV 1,2), 1000 testwells
SRA 46202/1000
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Reagent Set for Broad bean wilt virus 1 and 2 (BBWV 1,2), 5000 testwells
SRA 46202/5000
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