ELISA Reagent Set for Alfalfa mosaic virus (AMV)
Tech Note: Sample Extraction Buffer Change
Alternate Protocol: Alternate Extraction Buffer
This ELISA test is a qualitative serological assay for the detection of Alfalfa mosaic virus (AMV) in ornamental and vegetable leaves. AMV is a member of the Alfamovirus genus known for their bacilliform, with a constant diameter of 18 nm, viral particles.
Click here for other product documents such as the User Guide or Buffer Formulations.
Included:
- Capture antibody
- Detection antibody, bottle A
- Alkaline phosphatase enzyme conjugate, bottle B
- RUB6 enzyme conjugate diluent, 1X ready to use
- 96-well microtiter plates, solid* (*strip in single plate set)
- User Guide
- Tech Note
Required but Not Included:
- Buffer Pack (ACC 00333)
- Carbonate coating buffer, 10X concentrate
- PNP substrate buffer, 5X concentrate
- PNP substrate tablets, 5 mg each
- PBST buffer, powder
- General extract buffer
- TWEEN® 20
- Buffer Formulations from Chemicals
- Buffer Instructions from Powders and Concentrates
- Logsheet
For a full list of included items, including sizes, please see the Content List.
TWEEN® is a registered trademark of Croda International PLC.
Validation Report Summary
Test Label: Alkaline Phosphatase |
Full Validation Report |
| Analytical Sensitivity |
|---|
| Limit of detection: 1:729,000 dilution of infected tissue (pathogen titer unknown) |
| Isolate Names / Geographic Strain Locations | ||
|---|---|---|
| AMV-Arl 1 (WI, USA) (Soybean) | AMV-Arl 3 (WI, USA) (Soybean) | |
| AMV-BM 3 (WI, USA) (Kura) | AMV-K2 (WI, USA) (Kura) | |
| AMV-K5 (WI, USA) (Kura) | AMV-Lan 2 (WI, USA) (Soybean) | |
| AMV-OBT2 (WA, USA) (C. quinoa) | AMV PV-0040 | |
| AMV PV-0196 (Potato) | AMV PV-0779 (Germany) | |
| AMV-WI-2 (WA, USA) (Bean) | AMV-WM 1 (WI, USA) (Soybean) | |
| AMV-WM 2 (WI, USA) (Soybean) | AMV-YSC 4 (WI, USA) (Kura) | |
| AMV-0605 103 (WI, USA) (Soybean) | ||
| Cross-reactions | |
|---|---|
| None Known | |
For a list of validated hosts, please see the Full Validation Report.
| Option | Unit | Purchase |
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Reagent Set for Alfalfa mosaic virus (AMV), 96 testwells
SRA 87601/0096
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Shown in cart
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Reagent Set for Alfalfa mosaic virus (AMV), 500 testwells
SRA 87601/0500
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Shown in cart
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Reagent Set for Alfalfa mosaic virus (AMV), 1000 testwells
SRA 87601/1000
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Shown in cart
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Reagent Set for Alfalfa mosaic virus (AMV), 5000 testwells
SRA 87601/5000
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Shown in cart
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