ELISA Reagent Set for Alfalfa mosaic virus (AMV)

Tech Note: Sample Extraction Buffer Change

Alternate Protocol: Alternate Extraction Buffer

This ELISA test is a qualitative serological assay for the detection of Alfalfa mosaic virus (AMV) in ornamental and vegetable leaves. AMV is a member of the Alfamovirus genus known for their bacilliform, with a constant diameter of 18 nm, viral particles.

Click here for other product documents such as the User Guide or Buffer Formulations.

Included:

  • Capture antibody
  • Detection antibody, bottle A
  • Alkaline phosphatase enzyme conjugate, bottle B
  • RUB6 enzyme conjugate diluent, 1X ready to use
  • 96-well microtiter plates, solid* (*strip in single plate set)
  • User Guide
  • Tech Note

Required but Not Included:

For a full list of included items, including sizes, please see the Content List.

TWEEN® is a registered trademark of Croda International PLC.

Validation Report Summary

Test Label: Alkaline Phosphatase
Test Format: Compound-ELISA
Capture Reagent: Polyclonal
Detection Reagent: Monoclonal

Full Validation Report


Analytical Sensitivity
Limit of detection: 1:729,000 dilution of infected tissue (pathogen titer unknown)

Isolate Names / Geographic Strain Locations
AMV-Arl 1 (WI, USA) (Soybean) AMV-Arl 3 (WI, USA) (Soybean)
AMV-BM 3 (WI, USA) (Kura) AMV-K2 (WI, USA) (Kura)
AMV-K5 (WI, USA) (Kura) AMV-Lan 2 (WI, USA) (Soybean)
AMV-OBT2 (WA, USA) (C. quinoa) AMV PV-0040
AMV PV-0196 (Potato) AMV PV-0779 (Germany)
AMV-WI-2 (WA, USA) (Bean) AMV-WM 1 (WI, USA) (Soybean)
AMV-WM 2 (WI, USA) (Soybean) AMV-YSC 4 (WI, USA) (Kura)
AMV-0605 103 (WI, USA) (Soybean)

Cross-reactions
None Known

For a list of validated hosts, please see the Full Validation Report.

Option Unit Purchase
Reagent Set for Alfalfa mosaic virus (AMV), 96 testwells
SRA 87601/0096
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Reagent Set for Alfalfa mosaic virus (AMV), 500 testwells
SRA 87601/0500
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Reagent Set for Alfalfa mosaic virus (AMV), 1000 testwells
SRA 87601/1000
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Reagent Set for Alfalfa mosaic virus (AMV), 5000 testwells
SRA 87601/5000
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