ELISA Test Kits for AMV (Alfalfa mosaic virus)
Tech Note: AMV ELISA Protocol Change and Product Improvement
This ELISA test is a qualitative serological assay for the detection of Alfalfa mosaic virus (AMV) in ornamental and vegetable crops. AMV is a member of the Alfamovirus genus known for their bacilliform, with a constant diameter of 18 nm, viral particles.
If you would prefer to add a field-deployable assay to your testing toolkit, please check out Agdia's AMV ImmunoStrips®
Click here for other product documents such as the Reagent Set User Guide or PathoScreen Kit User Guide, click on the Documents tab for the full list.
Choose from the following formats (see the Included Items tab for full details):
- Reagent Set: Includes coating antibody, enzyme conjugate, and uncoated 96-well plate(s). Popular choice for established high-throughput laboratories.
- PathoScreen Complete Kit: Includes pre-coated 96-well plate(s), enzyme conjugate, positive control, and all required buffers. Easiest format to get started with ELISA and requires the least amount of time and labor to run.
- Capture Antibody: Only includes capture antibody. This is a partial component which is not usually ordered by itself.
- Enzyme Conjugate: Only includes enzyme conjugate and 96-well plate(s). This is a partial component which is not usually ordered by itself.
Reagent Set
Included in Reagent Set:
- Capture antibody
- Alkaline phosphatase enzyme conjugate
- 96-well microtiter plates, solid* (*strip in single plate set)
- Reagent Set User Guide
- Tech Note
Required but Not Included:
- Buffer Pack (ACC 00111)
- Carbonate coating buffer, 10X concentrate
- PNP substrate buffer, 5X concentrate
- PNP substrate tablets, 5 mg each
- PBST buffer, powder
- ECI buffer, 5X concentrate
- General extract buffer
- TWEEN® 20
- Buffer Formulations from Chemicals
- Logsheet
PathoScreen Kit
Included in PathoScreen Kit:
- Antibody-coated 96-well microtiter plates
- Alkaline phosphatase enzyme conjugate
- ECI buffer, 5X concentrate
- PNP substrate tablets, 5 mg each
- PNP substrate buffer, 5X concentrate
- Positive control
- PBST buffer, 20X concentrate, 50 mL
- TWEEN® 20
- General extract buffer, GEB
- PathoScreen User Guide
- Tech Note
Required but Not Included:
Coating Antibody
Included in Coating Antibody:
- Capture antibody
- 96-well microtiter plates, solid
Required but Not Included:
- Only a partial component; see above for complete options
Enzyme Conjugate
Included in Enzyme Conjugate:
- Alkaline phosphatase enzyme conjugate
- Reagent Set User Guide
- Tech Note
Required but Not Included:
- Only a partial component; see above for complete options
For a full list of included items, including sizes, please see the Content List.
TWEEN® is a registered trademark of Croda International PLC.
Validation Report Summary
Test Label: Alkaline Phosphatase |
Full Validation Report |
| Analytical Sensitivity |
|---|
| Analytical Sensitivity: 83.3% sensitive between 1000 ng/mL and 10 ng/mL. (n=18) |
| Limit of detection: 100% detection rate at 100 ng/mL with purified virus. (n=6) |
| Limit of detection: 50% detection rate at 10 ng/mL with purified virus. (n=6) |
| Isolate Names / Geographic Strain Locations | Analytical Sensitivity |
|---|---|
| AMV PV-0779 (Germany) | 1:1,000,000 dilution of infected tissue (pathogen titer unknown) |
| AMV PV-1282 (Czech Republic) | 1:1,000,000 dilution of infected tissue (pathogen titer unknown) |
| AMV-425M (ATCC® PV-92™) (WI, USA) | 1:10,000,000 dilution of infected tissue (pathogen titer unknown) |
| AMV-Aman (Argentina)1 | 1:100,000 dilution of infected tissue (pathogen titer unknown) |
| AMV-Ars2 (Italy) | 1:100,000 dilution of infected tissue (pathogen titer unknown) |
| AMV-ASdE (Argentina)1 | 1:100,000 dilution of infected tissue (pathogen titer unknown) |
| AMV-BSdE (Argentina)1 | 1:100,000 dilution of infected tissue (pathogen titer unknown) |
| AMV-Chiba1 (MAFF# 104001) (Japan) | 1:1,000,000 dilution of infected tissue (pathogen titer unknown) |
| AMV-Nevq (Argentina)1 | 1:100,000 dilution of infected tissue (pathogen titer unknown) |
| AMV-P (Kanagawa PP84-1) (MAFF# 104002) (Japan) | 1:1,000,000 dilution of infected tissue (pathogen titer unknown) |
| AMV-PS (Argentina)1 | 1:100,000 dilution of infected tissue (pathogen titer unknown) |
| AMV-S40 (ATCC® PV-848™) (Australia) | 1:100,000,000 dilution of infected tissue (pathogen titer unknown) |
1Isolates provided by Instituto de Patología Vegetal (IPAVE)
| Cross-reactions | |
|---|---|
| None Known | |
For a list of validated hosts, please see the Full Validation Report.
| Option | Unit | Purchase |
|---|---|---|
|
AMV Reagent Set, 96 Testwells
SRA 87601/0096
|
Shown in cart
|
|
|
AMV Reagent Set, 500 Testwells
SRA 87601/0500
|
Shown in cart
|
|
|
AMV Reagent Set, 1000 Testwells
SRA 87601/1000
|
Shown in cart
|
|
|
AMV Reagent Set, 5000 Testwells
SRA 87601/5000
|
Shown in cart
|
|
|
AMV PathoScreen® Complete Kit, 96 Testwells
PSA 87601/0096
|
Shown in cart
|
|
|
AMV PathoScreen® Complete Kit, 288 Testwells
PSA 87601/0288
|
Shown in cart
|
|
|
AMV PathoScreen® Complete Kit, 480 Testwells
PSA 87601/0480
|
Shown in cart
|