ELISA Test Kits for AMV (Alfalfa mosaic virus)
Tech Note: AMV ELISA Protocol Change and Product Improvement
This ELISA test is a qualitative serological assay for the detection of Alfalfa mosaic virus (AMV) in ornamental and vegetable crops. AMV is a member of the Alfamovirus genus known for their bacilliform, with a constant diameter of 18 nm, viral particles.
If you would prefer to add a field-deployable assay to your testing toolkit, please check out Agdia's AMV ImmunoStrips®
Click here for other product documents such as the Reagent Set User Guide or PathoScreen Kit User Guide, click on the Documents tab for the full list.
See below for a summary of our ELISA format offerings. Not all formats are available for every test. See the Included Items tab for full details.
- Reagent Set: Includes coating antibody, enzyme conjugate, and uncoated 96-well plate(s). Popular choice for established high-throughput laboratories.
- PathoScreen Complete Kit: Includes pre-coated 96-well plate(s), enzyme conjugate, positive control, and all required buffers. Easiest format to get started with ELISA and requires the least amount of time and labor to run.
- Capture Antibody: Only includes capture antibody. This is a partial component which is not usually ordered by itself.
- Enzyme Conjugate: Only includes enzyme conjugate and 96-well plate(s). This is a partial component which is not usually ordered by itself.
Reagent Set
Included in Reagent Set:
- Capture antibody
- Alkaline phosphatase enzyme conjugate
- 96-well microtiter plates, solid* (*strip in single plate set)
- Reagent Set User Guide
- Tech Note
Required but Not Included:
- Buffer Pack (ACC 00111)
- Carbonate coating buffer, 10X concentrate
- PNP substrate buffer, 5X concentrate
- PNP substrate tablets, 5 mg each
- PBST buffer, powder
- ECI buffer, 5X concentrate
- General extract buffer
- TWEEN® 20
- Buffer Formulations from Chemicals
- Logsheet
PathoScreen Kit
Included in PathoScreen Kit:
- Antibody-coated 96-well microtiter plates
- Alkaline phosphatase enzyme conjugate
- ECI buffer, 5X concentrate
- PNP substrate tablets, 5 mg each
- PNP substrate buffer, 5X concentrate
- Positive control
- PBST buffer, 20X concentrate, 50 mL
- TWEEN® 20
- General extract buffer, GEB
- PathoScreen User Guide
- Tech Note
Required but Not Included:
Coating Antibody
Included in Coating Antibody:
- Capture antibody
- 96-well microtiter plates, solid
Required but Not Included:
- Only a partial component; see above for complete options
Enzyme Conjugate
Included in Enzyme Conjugate:
- Alkaline phosphatase enzyme conjugate
- Reagent Set User Guide
- Tech Note
Required but Not Included:
- Only a partial component; see above for complete options
For a full list of included items, including sizes, please see the Content List.
TWEEN® is a registered trademark of Croda International PLC.
Validation Report Summary
Test Label: Alkaline Phosphatase |
Full Validation Report |
| Analytical Sensitivity |
|---|
| Analytical Sensitivity: 83.3% sensitive between 1000 ng/mL and 10 ng/mL. (n=18) |
| Limit of detection: 100% detection rate at 100 ng/mL with purified virus. (n=6) |
| Limit of detection: 50% detection rate at 10 ng/mL with purified virus. (n=6) |
| Isolate Names / Geographic Strain Locations | Analytical Sensitivity |
|---|---|
| AMV PV-0779 (Germany) | 1:1,000,000 dilution of infected tissue (pathogen titer unknown) |
| AMV PV-1282 (Czech Republic) | 1:1,000,000 dilution of infected tissue (pathogen titer unknown) |
| AMV-425M (ATCC® PV-92™) (WI, USA) | 1:10,000,000 dilution of infected tissue (pathogen titer unknown) |
| AMV-Aman (Argentina)1 | 1:100,000 dilution of infected tissue (pathogen titer unknown) |
| AMV-Ars2 (Italy) | 1:100,000 dilution of infected tissue (pathogen titer unknown) |
| AMV-ASdE (Argentina)1 | 1:100,000 dilution of infected tissue (pathogen titer unknown) |
| AMV-BSdE (Argentina)1 | 1:100,000 dilution of infected tissue (pathogen titer unknown) |
| AMV-Chiba1 (MAFF# 104001) (Japan) | 1:1,000,000 dilution of infected tissue (pathogen titer unknown) |
| AMV-Nevq (Argentina)1 | 1:100,000 dilution of infected tissue (pathogen titer unknown) |
| AMV-P (Kanagawa PP84-1) (MAFF# 104002) (Japan) | 1:1,000,000 dilution of infected tissue (pathogen titer unknown) |
| AMV-PS (Argentina)1 | 1:100,000 dilution of infected tissue (pathogen titer unknown) |
| AMV-S40 (ATCC® PV-848™) (Australia) | 1:100,000,000 dilution of infected tissue (pathogen titer unknown) |
1Isolates provided by Instituto de Patología Vegetal (IPAVE)
| Cross-reactions | |
|---|---|
| None Known | |
For a list of validated hosts, please see the Full Validation Report.
| Option | Unit | Purchase |
|---|---|---|
|
AMV Reagent Set, 96 Testwells
SRA 87601/0096
|
Shown in cart
|
|
|
AMV Reagent Set, 500 Testwells
SRA 87601/0500
|
Shown in cart
|
|
|
AMV Reagent Set, 1000 Testwells
SRA 87601/1000
|
Shown in cart
|
|
|
AMV Reagent Set, 5000 Testwells
SRA 87601/5000
|
Shown in cart
|
|
|
AMV PathoScreen® Complete Kit, 96 Testwells
PSA 87601/0096
|
Shown in cart
|
|
|
AMV PathoScreen® Complete Kit, 288 Testwells
PSA 87601/0288
|
Shown in cart
|
|
|
AMV PathoScreen® Complete Kit, 480 Testwells
PSA 87601/0480
|
Shown in cart
|