ELISA Reagent Set for Acidovorax avenae subsp. citrulli (Aac)
Tech Note: Extraction Buffer and Enzyme Conjugate Diluent Change
Alternate Protocol: Alternate Buffers
This ELISA test is a qualitative serological assay for the detection of Acidovorax avenae subsp. citrulli (Aac), the causal agent of bacterial fruit blotch, in cucurbit leaves, fruit, bacterial cultures, and seedlings. Aac is divided into two distinct groups. Group I strains are associated with non-watermelon plants while Group II strains are associated with watermelon plants.
If you would prefer to add a field-deployable assay to your testing toolkit, please check out Agdia's Aac ImmunoStrips®.
Click here for other product documents such as the User Guide or Buffer Formulations.
Included:
- Capture antibody
- Alkaline phosphatase enzyme conjugate
- RUB6 enzyme conjugate diluent, 1X ready to use
- 96-well microtiter plates, solid* (*strip in single plate set)
- User Guide
- Tech Note
Required but Not Included:
- Buffer Pack (ACC 00333)
- Carbonate coating buffer, 10X concentrate
- PNP substrate buffer, 5X concentrate
- PNP substrate tablets, 5 mg each
- PBST buffer, powder
- General extract buffer
- TWEEN® 20
- Buffer Formulations from Chemicals
- Buffer Instructions from Powders and Concentrates
- Logsheet
For a full list of included items, including sizes, please see the Content List.
TWEEN® is a registered trademark of Croda International PLC.
Validation Report Summary
Test Label: Alkaline Phosphatase |
Full Validation Report |
| Analytical Sensitivity |
|---|
| Limit of detection: 6x107 CFU/mL |
| Isolate Names / Geographic Strain Locations | |
|---|---|
| Aac Austrailia strain | Aac Brazil strain |
| Aac Canada strain | Aac China strain |
| Aac Spain strain | Aac Florida (USA) strain |
| Aac Gerogia (USA) strain | |
| Cross-reactions | |
|---|---|
| Ochrobactrum pseudogrignonense | |
For a list of validated hosts, please see the Full Validation Report.
| Option | Unit | Purchase |
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Reagent Set for Acidovorax avenae subsp. citrulli (Aac), 96 testwells
SRA 14800/0096
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Shown in cart
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Reagent Set for Acidovorax avenae subsp. citrulli (Aac), 500 testwells
SRA 14800/0500
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Shown in cart
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Reagent Set for Acidovorax avenae subsp. citrulli (Aac), 1000 testwells
SRA 14800/1000
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Shown in cart
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Reagent Set for Acidovorax avenae subsp. citrulli (Aac), 5000 testwells
SRA 14800/5000
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Shown in cart
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