ELISA Reagent Set for Acidovorax avenae subsp. citrulli (Aac)

Tech Note: Cross-Reaction

This ELISA test is a qualitative serological assay for the detection of Acidovorax avenae subsp. citrulli (Aac), the causal agent of bacterial fruit blotch, in cucurbit leaves, fruit, bacterial cultures, and seedlings. Aac is divided into two distinct groups. Group I strains are associated with non-watermelon plants while Group II strains are associated with watermelon plants.

If you would prefer to add a field-deployable assay to your testing toolkit, please check out Agdia's Aac ImmunoStrips®

Click here for other product documents such as the User Guide or Buffer Formulations.

Included:

  • Capture antibody
  • Alkaline phosphatase enzyme conjugate
  • RUB3 enzyme conjugate diluent
  • General extract buffer 2, GEB2
  • 96-well microtiter plates, solid* (*strip in single plate set)
  • User Guide
  • Tech Note

Required but Not Included:

For a full list of included items, including sizes, please see the Content List.

Validation Report Summary

Test Label: Alkaline Phosphatase
Test Format: Double Antibody Sandwich (DAS) ELISA
Capture Reagent: Monoclonal
Detection Reagent: Monoclonal

Full Validation Report


Analytical Sensitivity
Limit of detection: 6x107 CFU/mL

Isolate Names / Geographic Strain Locations
Aac Austrailia strain Aac Brazil strain
Aac Canada strain Aac China strain
Aac Spain strain Aac Florida (USA) strain
Aac Gerogia (USA) strain

Cross-reactions
Ochrobactrum pseudogrignonense

For a list of validated hosts, please see the Full Validation Report.

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Reagent Set for Acidovorax avenae subsp. citrulli (Aac), 96 testwells
SRA 14800/0096
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Reagent Set for Acidovorax avenae subsp. citrulli (Aac), 500 testwells
SRA 14800/0500
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Reagent Set for Acidovorax avenae subsp. citrulli (Aac), 1000 testwells
SRA 14800/1000
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Reagent Set for Acidovorax avenae subsp. citrulli (Aac), 5000 testwells
SRA 14800/5000
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