PCR Primer for Tospovirus Group
The Tospovirus Group PCR primers offer a sensitive diagnostic method to detect members of the Orthotospovirus genus of the Tospoviridae family. The primer sequences are based on conserved genome regions and can detect characterized and unassigned members of the Orthotospovirus genus.
This test can be used as an aid for identification of viral etiology of unknown plant diseases, test for known viruses when other tests are not available and can be used to confirm results from other test methods. For a full list of confirmed and predicted reactions, click here.
The Tospovirus Group PCR primer product includes a lyophilized mix of forward and reverse primers, PCR quality water and detailed instructions.
Disclaimer of License: This product is optimized for use in the Polymerase Chain Reaction (PCR) process covered by patents owned by Hoffmann-La Roche, Inc. and F. Hoffmann-La Roche Ltd. Purchase of this product does not include the license under these patents to use the PCR process. This product is recommended for use in conjunction with an authorized thermal cycler.
Click here for other product documents such as the User Guide or Validation Report.
Included:
- Lyophilized forward and reverse primers
- Sterile PCR quality water
- User Guide
Required but Not Included:
- RNA Extraction Materials
- Reverse Transcriptase and PCR Materials
- Thermal cycler
- Gel electrophoreseis Materials
For a full list of included items, including sizes, please see the Content List.
Validation Report Summary
Test Format: PCR | Full Validation Report |
| Analytical Sensitivity |
|---|
| Limit of detection: N/A |
| Species Detected1 | |
|---|---|
| Virus Name | Binomial Species Name |
| Alstroemeria necrotic streak virus (ANSV) | Orthotospovirus alstroemerinecrosis |
| Alstroemeria yellow spot virus (AYSV)2 | Orthotospovirus alstroemeriflavi |
| Bean necrotic mosaic virus (BeNMV)2 | Orthotospovirus phaseolinecrotessellati |
| Calla lily chlorotic spot virus (CCSV)2 | Orthotospovirus callaflavi |
| Capsicum chlorosis virus (CaCV) | Orthotospovirus capsiciflavi |
| Chrysanthemum stem necrosis virus (CSNV) | Orthotospovirus chrysanthinecrocaulis |
| Groundnut bud necrosis virus (GBNV)2,4 | Orthotospovirus arachinecrosis |
| Groundnut chlorotic fan-spot virus (GCFSV)2 | Orthotospovirus arachiflavi |
| Groundnut ringspot virus (GRSV) | Orthotospovirus arachianuli |
| Hippeastrum chlorotic ringspot virus (HCRV)2 | Orthotospovirus hippeflavi |
| Impatiens necrotic spot virus (INSV) | Orthotospovirus impatiensnecromaculae |
| Iris yellow spot virus (IYSV) | Orthotospovirus iridimaculaflavi |
| Melon severe mosaic virus (MeSMV) | Orthotospovirus melotessellati |
| Melon yellow spot virus (MYSV) | Orthotospovirus meloflavi |
| Mulberry vein banding associated virus (MVBaV)2 | Orthotospovirus morivenae |
| Pepper chlorotic spot virus (PCSV)2 | Orthotospovirus capsicimaculaflavi |
| Polygonum ringspot virus (PolRSV)2 | Orthotospovirus polygonianuli |
| Soybean vein necrosis virus (SVNV) | Orthotospovirus glycininecrovenae |
| Tomato chlorotic spot virus (TCSV) | Orthotospovirus tomatoflavi |
| Tomato necrotic ringspot virus (ToNSV)2,3 | |
| Tomato necrotic spot-associated virus (TNSaV)2,3 | |
| Tomato spotted wilt virus (TSWV) | Orthotospovirus tomatomaculae |
| Tomato yellow fruit ring virus (TYFRV)2,3 | |
| Tomato yellow ring virus (TYRV)2 | Orthotospovirus tomatanuli |
| Tomato zonate spot virus (TZSV)2 | Orthotospovirus tomatozonae |
| Watermelon bud necrosis virus (WBNV)2 | Orthotospovirus citrullonecrosis |
| Watermelon silver mottle virus (WSMoV) | Orthotospovirus citrullomaculosi |
| Zucchini lethal chlorosis virus (ZLCV)2 | Orthotospovirus cucurbichlorosis |
1The list above represents viruses that have been shown to be detected by this group PCR test. It also represents viruses that may be detected based on in silico analysis. If you have confirmed detection of a predicted virus detection or a virus not on this list, please contact us. We would like to work with you to further validate detection capabilities.
2Predicted detection by in silico analysis only
3Tentative Orthotospovirus
4Peanut bud necrosis virus (PBNV) is now known as Groundnut bud necrosis virus (GBNV)
| Cross-reactions | |
|---|---|
| None known | |
For a list of validated hosts, please see the Full Validation Report.
| Additional Documents | ||
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