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PCR Primer for Tospovirus Group

Part Number:
PCR 98100/0025
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The Tospovirus Group PCR primers offer a sensitive diagnostic method to detect members of the Orthotospovirus genus of the Tospoviridae family. The primer sequences are based on conserved genome regions and can detect characterized and unassigned members of the Orthotospovirus genus.

This test can be used as an aid for identification of viral etiology of unknown plant diseases, test for known viruses when other tests are not available and can be used to confirm results from other test methods. For a full list of confirmed and predicted reactions, click here.

The Tospovirus Group PCR primer product includes a lyophilized mix of forward and reverse primers, PCR quality water and detailed instructions.

Disclaimer of License: This product is optimized for use in the Polymerase Chain Reaction (PCR) process covered by patents owned by Hoffmann-La Roche, Inc. and F. Hoffmann-La Roche Ltd. Purchase of this product does not include the license under these patents to use the PCR process. This product is recommended for use in conjunction with an authorized thermal cycler.

Click here for other product documents such as the User Guide or Validation Report.


  • Lyophilized forward and reverse primers
  • Sterile PCR quality water
  • User Guide

Required but Not Included:

  • RNA Extraction Materials
  • Reverse Transcriptase and PCR Materials
  • Thermal cycler
  • Gel electrophoreseis Materials

For a full list of included items, including sizes, please see the Content List.

Validation Report Summary

Test Format: PCR
Target Label: N/A
Internal Control: N/A

Full Validation Report

Analytical Sensitivity
Limit of detection: N/A

Species Detected1
Virus Name Binomial Species Name
Alstroemeria necrotic streak virus (ANSV) Orthotospovirus alstroemerinecrosis
Alstroemeria yellow spot virus (AYSV)2 Orthotospovirus alstroemeriflavi
Bean necrotic mosaic virus (BeNMV)2 Orthotospovirus phaseolinecrotessellati
Calla lily chlorotic spot virus (CCSV)2 Orthotospovirus callaflavi
Capsicum chlorosis virus (CaCV) Orthotospovirus capsiciflavi
Chrysanthemum stem necrosis virus (CSNV) Orthotospovirus chrysanthinecrocaulis
Groundnut bud necrosis virus (GBNV)2,4 Orthotospovirus arachinecrosis
Groundnut chlorotic fan-spot virus (GCFSV)2 Orthotospovirus arachiflavi
Groundnut ringspot virus (GRSV) Orthotospovirus arachianuli
Hippeastrum chlorotic ringspot virus (HCRV)2 Orthotospovirus hippeflavi
Impatiens necrotic spot virus (INSV) Orthotospovirus impatiensnecromaculae
Iris yellow spot virus (IYSV) Orthotospovirus iridimaculaflavi
Melon severe mosaic virus (MeSMV) Orthotospovirus melotessellati
Melon yellow spot virus (MYSV) Orthotospovirus meloflavi
Mulberry vein banding associated virus (MVBaV)2 Orthotospovirus morivenae
Pepper chlorotic spot virus (PCSV)2 Orthotospovirus capsicimaculaflavi
Polygonum ringspot virus (PolRSV)2 Orthotospovirus polygonianuli
Soybean vein necrosis virus (SVNV) Orthotospovirus glycininecrovenae
Tomato chlorotic spot virus (TCSV) Orthotospovirus tomatoflavi
Tomato necrotic ringspot virus (ToNSV)2,3
Tomato necrotic spot-associated virus (TNSaV)2,3
Tomato spotted wilt virus (TSWV) Orthotospovirus tomatomaculae
Tomato yellow fruit ring virus (TYFRV)2,3
Tomato yellow ring virus (TYRV)2 Orthotospovirus tomatanuli
Tomato zonate spot virus (TZSV)2 Orthotospovirus tomatozonae
Watermelon bud necrosis virus (WBNV)2 Orthotospovirus citrullonecrosis
Watermelon silver mottle virus (WSMoV) Orthotospovirus citrullomaculosi
Zucchini lethal chlorosis virus (ZLCV)2 Orthotospovirus cucurbichlorosis

1The list above represents viruses that have been shown to be detected by this group PCR test. It also represents viruses that may be detected based on in silico analysis. If you have confirmed detection of a predicted virus detection or a virus not on this list, please contact us. We would like to work with you to further validate detection capabilities.
2Predicted detection by in silico analysis only
3Tentative Orthotospovirus
4Peanut bud necrosis virus (PBNV) is now known as Groundnut bud necrosis virus (GBNV)

None known

For a list of validated hosts, please see the Full Validation Report.

  • Included Items
  • Technical Information
  • Documents