PCR Primer for Tospovirus Group
The Tospovirus Group PCR primers offer a sensitive diagnostic method to detect members of the Tospovirus genus of the Bunyaviridae family. The primer sequences are based on conserved genome regions and can detect characterized and unassigned members of the Tospovirus genus.
This test can be used as an aid for identification of viral etiology of unknown plant diseases, test for known viruses when other tests are not available and can be used to confirm results from other test methods. For a full list of confirmed and predicted reactions, click here.
The Tospovirus Group PCR primer product includes a lyophilized mix of forward and reverse primers, PCR quality water and detailed instructions.
Disclaimer of License: This product is optimized for use in the Polymerase Chain Reaction (PCR) process covered by patents owned by Hoffmann-La Roche, Inc. and F. Hoffmann-La Roche Ltd. Purchase of this product does not include the license under these patents to use the PCR process. This product is recommended for use in conjunction with an authorized thermal cycler.
This test reacts with a variety of viruses from the Tospovirus genus. The list below represents Tospoviruses that have been experimentally detected with our Tospovirus PCR and confirmed by sequencing. If you have confirmed detection of a Tospovirus not on this list, please contact us. For viruses predicted to be detected, click here.
|Capsicum chlorosis virus (CaCV)||Chrysanthemum stem necrosis virus (CSNV)|
|Groundnut ringspot virus (GRSV)||Impatiens necrotic spot virus (INSV)|
|Iris yellow spot virus (IYSV)||Soybean vein necrosis virus (SVNV)|
|Tomato chlorotic spot virus (TCSV)||Tomato spotted wilt virus (TSWV)|
|Watermelon silver mottle virus (WSMoV)|
This test reacts with a variety of viruses from the Tospovirus genus. The list below represents Tospoviruses that have not been experimentally detected, but based on in silico analysis, will likely be detected with our Tospovirus PCR. If you have samples of these pathogens below, please contact us.
|Bean necrotic mosaic virus (BeNMV)||Calla lily chlorotic spot virus (CCSV)|
|Groundnut bud necrosis virus (GBNV)||Melon severe mosaic virus (MeSMV)|
|Melon yellow spot virus (MYSV)||Peanut bud necrosis virus (PBNV)|
|Pepper chlorotic spot virus (PCSV)||Polygonum ringspot virus (PolRSV)|
|Tomato yellow ring virus (TYRV)||Watermelon bud necrosis virus (WBNV)|
|Zucchini lethal chlorosis virus (ZLCV)||Groundnut chlorotic fan-spot virus (GCFSV)*|
|Hippeastrum chlorotic ringspot virus (HCRV)*||Mulberry vein banding virus (MVBaV)*|
|Tomato necrotic spot-associated virus (TNSaV)*||Tomato yellow fruit ring virus (TYFRV)*|
|Tomato zonate spot virus (TZSV)*|
* Tentative Tospoviruses
- Lyophilized forward and reverse primers
- Sterile PCR quality water
- User Guide