PCR Primer for Tobamovirus
The Tobamovirus Group PCR primers offer a sensitive diagnostic method to detect members of the Tobamovirus genus of the Virgaviridae family. The primer sequences are based on conserved genome regions and can detect characterized and unassigned members of the Tobamovirus genus.
This test can be used as an aid for identification of viral etiology of unknown plant diseases, test for known viruses when other tests are not available and can be used to confirm results from other test methods. For a full list of confirmed and predicted reactions, click here.
The Tobamovirus Group PCR primer product includes a lyophilized mix of forward and reverse primers, PCR quality water and detailed instructions.
Disclaimer of License: This product is optimized for use in the Polymerase Chain Reaction (PCR) process covered by patents owned by Hoffmann-La Roche, Inc. and F. Hoffmann-La Roche Ltd. Purchase of this product does not include the license under these patents to use the PCR process. This product is recommended for use in conjunction with an authorized thermal cycler.
Click here for other product documents such as the User Guide or Validation Report.
Included:
- Lyophilized forward and reverse primers
- Sterile PCR quality water
- User Guide
Required but Not Included:
- RNA Extraction Materials
- Reverse Transcriptase and PCR Materials
- Thermal cycler
- Gel electrophoreseis Materials
For a full list of included items, including sizes, please see the Content List.
Validation Report Summary
Test Format: PCR | Full Validation Report |
| Analytical Sensitivity |
|---|
| Limit of detection: N/A |
| Species Detected1 | |
|---|---|
| Abutilon yellow mosaic virus (AbYMV)2,3 | African eggplant-associated virus (AEaV) |
| Bell pepper mottle virus (BPeMV) | Brugmansia mild mottle virus (BrMMV)2 |
| Cactus mild mottle virus (CMMoV)2 | Chili pepper mild mottle virus (CPMMoV)4 |
| Clitoria yellow mottle virus (CliYMMV)2 | Cucumber fruit mottle mosaic virus (CFMMV)2 |
| Cucumber green mottle mosaic virus (CGMMV) | Cucumber mottle virus (CuMoV)2 |
| Hibiscus latent Fort Pierce virus (HLFPV)2 | Hibiscus latent Singapore virus (HLSV)2 |
| Hoya chlorotic spot virus (HoCSV)2,3 | Kyuri green mild mottle virus (KGMMV) |
| Maracuja mosaic virus (MarMV)2 | Obuda pepper virus (OBPV) |
| Odontoglossum ringspot virus (ORSV) | Paprika mild mottle virus (PaMMV) |
| Passion fruit mosaic virus (PafMV)2 | Pepper mild mottle virus (PMMoV) |
| Piper chlorosis virus (PChV)5 | Plumeria mosaic virus (PluMV)2 |
| Rattail cactus necrosis-associated virus (RCNAV)2 | Rehmannia mosaic virus (ReMV) |
| Ribgrass mosaic virus (RMV) | Streptocarpus flower break virus (SFBV)2 |
| Sunn-hemp mosaic virus (SHMV) | Tobacco mild green mosaic virus (TMGMV) |
| Tobacco mosaic virus (TMV) | Tomato brown rugose fruit virus (ToBRFV) |
| Tomato mosaic virus (ToMV) | Tomato mottle mosaic virus (ToMMV) |
| Tropical soda apple mosaic virus (TSAMV) | Turnip vein clearing virus (TVCV) |
| Wasabi mottle virus (WMoV)2 | Watermelon green mottle mosaic virus (WGMMV)2,6 |
| Yellow tailflower mild mottle virus (YTMMV) | Youcai mosaic virus (YoMV) |
| Zucchini green mottle mosaic virus (ZGMMV) | |
1The list above represents viruses that have been shown to be detected by this group PCR test. It also represents viruses that may be detected based on in silico analysis. If you have confirmed detection of a predicted virus detection or a virus not on this list, please contact us. We would like to work with you to further validate detection capabilities.
2Predicted detection by in silico analysis only
3Described by ICTV as a related, unclassified Tobamovirus
4Confirmed to detect Chili pepper mild mottle virus (CPMMoV), a possible novel Tobamovirus.
5Confirmed to detect Piper chlorosis virus (PChV), a possible novel Tobamovirus.
6
Predicted to detect Watermelon green mottle mosaic virus (WGMMV), a possible novel Tobamovirus.
| Cross-reactions | |
|---|---|
| None known | |
For a list of validated hosts, please see the Full Validation Report.
| Additional Documents | ||
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