PCR Primer for Tobamovirus
The Tobamovirus Group PCR primers offer a sensitive diagnostic method to detect members of the Tobamovirus genus of the Virgaviridae family. The primer sequences are based on conserved genome regions and can detect characterized and unassigned members of the Tobamovirus genus.
This test can be used as an aid for identification of viral etiology of unknown plant diseases, test for known viruses when other tests are not available and can be used to confirm results from other test methods. For a full list of confirmed and predicted reactions, click here.
The Tobamovirus Group PCR primer product includes a lyophilized mix of forward and reverse primers, PCR quality water and detailed instructions.
Disclaimer of License: This product is optimized for use in the Polymerase Chain Reaction (PCR) process covered by patents owned by Hoffmann-La Roche, Inc. and F. Hoffmann-La Roche Ltd. Purchase of this product does not include the license under these patents to use the PCR process. This product is recommended for use in conjunction with an authorized thermal cycler.
Click here for other product documents such as the User Guide or Validation Report.
Included:
- Lyophilized forward and reverse primers
- Sterile PCR quality water
- User Guide
Required but Not Included:
- RNA Extraction Materials
- Reverse Transcriptase and PCR Materials
- Thermal cycler
- Gel electrophoreseis Materials
For a full list of included items, including sizes, please see the Content List.
Validation Report Summary
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Test Format: PCR |
Full Validation Report |
| Analytical Sensitivity |
|---|
| Limit of detection: N/A |
| Species Detected1 | |
|---|---|
| Virus Name | Species Name |
| Abutilon yellow mosaic virus (AbYMV)2,3 | N/A |
| African eggplant-associated virus (AEaV)4 | N/A |
| Bell pepper mottle virus (BPeMV) | Tobamovirus maculacapsici |
| Brugmansia latent virus (BrLV)5 | N/A |
| Brugmansia mild mottle virus (BrMMV)2 | Tobamovirus brugmansiae |
| Cactus mild mottle virus (CMMoV)2 | Tobamovirus cacti |
| Chili pepper mild mottle virus (CPMMoV)6 | N/A |
| Clitoria yellow mottle virus (CliYMMV)2 | Tobamovirus clitoriae |
| Cucumber fruit mottle mosaic virus (CFMMV) | Tobamovirus maculafructi |
| Cucumber green mottle mosaic virus (CGMMV) | Tobamovirus viridimaculae |
| Cucumber mottle virus (CMoV) | Tobamovirus cucumeris |
| Hibiscus latent Fort Pierce virus (HLFPV)2 | Tobamovirus fortpiercense |
| Hibiscus latent Singapore virus (HLSV)2 | Tobamovirus singaporense |
| Hoya chlorotic spot virus (HoCSV)2,3 | N/A |
| Kyuri green mottle mosaic virus (KGMMV) | Tobamovirus kyuri |
| Maracuja mosaic virus (MarMV) | Tobamovirus maracujae |
| Obuda pepper virus (ObPV) | Tobamovirus obudae |
| Odontoglossum ringspot virus (ORSV) | Tobamovirus odontoglossi |
| Paprika mild mottle virus (PaMMV) | Tobamovirus paprikae |
| Passion fruit mosaic virus (PafMV)2 | Tobamovirus passiflorae |
| Pepper mild mottle virus (PMMoV) | Tobamovirus capsici |
| Piper chlorosis virus (PChV)7 | N/A |
| Plumeria mosaic virus (PluMV)2 | Tobamovirus plumeriae |
| Rattail cactus necrosis-associated virus (RCNAV)2 | Tobamovirus muricaudae |
| Rehmannia mosaic virus (ReMV) | Tobamovirus rehmanniae |
| Ribgrass mosaic virus (RMV) | Tobamovirus plantagonis |
| Scopolia mild mottle virus (SMMoV)8 | N/A |
| Streptocarpus flower break virus (SFBV) | Tobamovirus streptocarpi |
| Sunn-hemp mosaic virus (SHMV) | Tobamovirus crotalariae |
| Tobacco mild green mosaic virus (TMGMV) | Tobamovirus mititessellati |
| Tobacco mosaic virus (TMV) | Tobamovirus tabaci |
| Tomato brown rugose fruit virus (ToBRFV) | Tobamovirus fructirugosum |
| Tomato mosaic virus (ToMV) | Tobamovirus tomatotessellati |
| Tomato mottle mosaic virus (ToMMV) | Tobamovirus maculatessellati |
| Tropical soda apple mosaic virus (TSAMV) | Tobamovirus tropici |
| Turnip vein-clearing virus (TVCV) | Tobamovirus rapae |
| Wasabi mottle virus (WMoV) | Tobamovirus wasabi |
| Watermelon green mottle mosaic virus (WGMMV)2,9 | N/A |
| Yellow tailflower mild mottle virus (YTMMV) | Tobamovirus anthocercis |
| Youcai mosaic virus (YoMV) | Tobamovirus youcai |
| Zucchini green mottle mosaic virus (ZGMMV) | Tobamovirus cucurbitae |
1The list above represents viruses that have been shown to be detected by this group PCR test.
It also represents viruses that may be detected based on in silico analysis. If you have confirmed
detection of a predicted virus detection or a virus not on this list, please contact us. We would like to work
with you to further validate detection capabilities.
2Predicted detection by in silico analysis only
3Described by ICTV as a related, unclassified Tobamovirus
4African eggplant-associated virus (AEaV) has been reported to be a possible novel Tobamovirus.
5Brugmansia latent virus (BrLV) has been reported to be a possible novel Tobamovirus.
6Chili pepper mild mottle virus (CPMMoV) has been reported to be a possible novel Tobamovirus.
7Piper chlorosis virus (PChV) has been reported to be a possible novel Tobamovirus.
8Scopolia mild mottle virus (SMMoV) has been reported to be a
possible novel Tobamovirus.
9Watermelon green mottle mosaic virus (WGMMV) has been reported to be a
possible novel Tobamovirus.
| Cross-reactions | |
|---|---|
| None known | |
For a list of validated hosts, please see the Full Validation Report.
| Additional Documents | ||
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