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PCR Primer for Dianthovirus Group

Part Number:
PCR 95000/0025
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The Dianthovirus Group PCR primers offer a sensitive diagnostic method to detect members of the Dianthovirus genus of the Tombusviridae family. The primer sequences are based on conserved genome regions and can detect characterized and unassigned members of the Dianthovirus genus.

This test can be used as an aid for identification of viral etiology of unknown plant diseases, test for known viruses when other tests are not available and can be used to confirm results from other test methods. For a full list of confirmed and predicted reactions, click here.

The Dianthovirus Group PCR primer product includes a lyophilized mix of forward and reverse primers, PCR quality water and detailed instructions.

Disclaimer of License: This product is optimized for use in the Polymerase Chain Reaction (PCR) process covered by patents owned by Hoffmann-La Roche, Inc. and F. Hoffmann-La Roche Ltd. Purchase of this product does not include the license under these patents to use the PCR process. This product is recommended for use in conjunction with an authorized thermal cycler.

Click here for other product documents such as the User Guide or Validation Report.

Included:

  • Lyophilized forward and reverse primers
  • Sterile PCR quality water
  • User Guide

Required but Not Included:

  • RNA Extraction Materials
  • Reverse Transcriptase and PCR Materials
  • Thermal cycler
  • Gel electrophoreseis Materials

For a full list of included items, including sizes, please see the Content List.

Validation Report Summary

Test Format: PCR
Target Label: N/A
Internal Control: N/A

Full Validation Report

Analytical Sensitivity
Limit of detection: N/A

Species Detected1
Virus Name Species Name
Carnation ringspot virus (CRSV) Dianthovirus dianthi
Red clover necrotic mosaic virus (RCNMV) Dianthovirus trifolii
Rice virus X (RVX)2,3 N/A
Sweet clover necrotic mosaic virus (SCNMV) Dianthovirus meliloti

1The list above represents viruses that have been shown to be detected by this group PCR test. It also represents viruses that may be detected based on in silico analysis. If you have confirmed detection of a predicted virus detection or a virus not on this list, please contact us. We would like to work with you to further validate detection capabilities.
2Predicted detection by in silico analysis only
3Rice virus X is a tentative Dianthovirus.

Cross-reactions
None Known

For a list of validated hosts, please see the Full Validation Report.

  • Included Items
  • Technical Information
  • Documents