PCR Primer for Carmovirus-Pelarspovirus
The Carmovirus-Pelarspovirus Group PCR primers offer a sensitive diagnostic method to detect members of the Alphacarmovirus, Betacarmovirus, Gammacarmovirus, and Pelarspovirus genera of the Tombusviridae family. The primer sequences are based on conserved genome regions and can detect characterized and unassigned members of the Alphacarmovirus, Betacarmovirus, Gammacarmovirus, and Pelarspovirus genera.
This test can be used as an aid for identification of viral etiology of unknown plant diseases, test for known viruses when other tests are not available and can be used to confirm results from other test methods. For a full list of confirmed and predicted reactions, click here.
The Carmovirus-Pelarspovirus Group PCR primer product includes a lyophilized mix of forward and reverse primers, PCR quality water and detailed instructions.
Disclaimer of License: This product is optimized for use in the Polymerase Chain Reaction (PCR) process covered by patents owned by Hoffmann-La Roche, Inc. and F. Hoffmann-La Roche Ltd. Purchase of this product does not include the license under these patents to use the PCR process. This product is recommended for use in conjunction with an authorized thermal cycler.
Click here for other product documents such as the User Guide or Validation Report.
Included:
- Lyophilized forward and reverse primers
- Sterile PCR quality water
- User Guide
Required but Not Included:
- RNA Extraction Materials
- Reverse Transcriptase and PCR Materials
- Thermal cycler
- Gel electrophoreseis Materials
For a full list of included items, including sizes, please see the Content List.
Validation Report Summary
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Test Format: PCR |
Full Validation Report |
| Analytical Sensitivity |
|---|
| Limit of detection: N/A |
| Species Detected1 | |
|---|---|
| Virus Name | Species Name |
| Angelonia flower break virus (AnFBV) | Alphacarmovirus angeloniae |
| Calibrachoa mottle virus (CbMV) | Alphacarmovirus calibrachoae |
| Cardamine chlorotic fleck virus (CCFV) | Betacarmovirus cardaminis |
| Carnation mottle virus (CarMV) | Alphacarmovirus dianthi |
| Clematis chlorotic mottle virus (ClCMV) | Pelarspovirus clematis |
| Cowpea mottle virus (CPMoV) | Gammacarmovirus vignae |
| Elderberry latent virus (ELV) | Pelarspovirus sambuci |
| Hibiscus chlorotic ringspot virus (HCRSV) | Betacarmovirus hibisci |
| Honeysuckle ringspot virus (HRSV)2 | Alphacarmovirus lonicerae |
| Japanese iris necrotic ring virus (JINRV)2 | Betacarmovirus iridis |
| Melon necrotic spot virus (MNSV) | Gammacarmovirus melonis |
| Nootka lupine vein clearing virus (NLVCV)2 | Alphacarmovirus lupini |
| Pea stem necrosis virus (PSNV)2 | Gammacarmovirus pisi |
| Pelargonium chlorotic ring pattern virus (PCRPV) | Pelarspovirus chloropelargonii |
| Pelargonium flower break virus (PFBV) | Alphacarmovirus pelargonii |
| Pelargonium line pattern virus (PLPV) | Pelarspovirus lineapelargonii |
| Pelargonium ringspot virus (PelRSV) | Pelarspovirus anulopelargonii |
| Rosa rugosa leaf distortion virus (RRLDV)2 | Pelarspovirus rosae |
| Saguaro cactus virus (SgCV) | Alphacarmovirus cacti |
| Soybean yellow mottle mosaic virus (SYMMV)2 | Gammacarmovirus glycinis |
| Turnip crinkle virus (TCV) | Betacarmovirus brassicae |
1The list above represents viruses that have been shown to be detected by this group PCR test.
It also represents viruses that may be detected based on in silico analysis. If you have confirmed
detection of a predicted virus detection or a virus not on this list, please contact us. We would like to work
with you to further validate detection capabilities.
2Predicted detection by in silico analysis only
| Cross-reactions | |
|---|---|
| Galinsoga mosaic virus (GAMV)1 | Gallantivirus galinsogae |
| Maize chlorotic mottle virus (MCMV) | Machlomovirus zeae |
| Tobacco necrosis virus A (TNV-A) | Alphanecrovirus nicotianae |
| Trailing lespedeza virus 1 (TLV1)1 | Tralespevirus lespedezae |
1Predicted detection by in silico analysis only
For a list of validated hosts, please see the Full Validation Report.
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