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PCR Primer for Carlavirus

Part Number:
PCR 93000/0025
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The Carlavirus Group PCR primers offer a sensitive diagnostic method to detect members of the Carlavirus genus of the Betaflexiviridae family. The primer sequences are based on conserved genome regions and can detect characterized and unassigned members of the Carlavirus genus.

This test can be used as an aid for identification of viral etiology of unknown plant diseases, test for known viruses when other tests are not available and can be used to confirm results from other test methods. For a full list of confirmed and predicted reactions, click here.

The Carlavirus Group PCR primer product includes a lyophilized mix of forward and reverse primers, PCR quality water and detailed instructions.

Disclaimer of License: This product is optimized for use in the Polymerase Chain Reaction (PCR) process covered by patents owned by Hoffmann-La Roche, Inc. and F. Hoffmann-La Roche Ltd. Purchase of this product does not include the license under these patents to use the PCR process. This product is recommended for use in conjunction with an authorized thermal cycler.

Click here for other product documents such as the User Guide or Validation Report.

Included:

  • Lyophilized forward and reverse primers
  • Sterile PCR quality water
  • User Guide

Required but Not Included:

  • RNA Extraction Materials
  • Reverse Transcriptase and PCR Materials
  • Thermal cycler
  • Gel electrophoreseis Materials

For a full list of included items, including sizes, please see the Content List.

Validation Report Summary

Test Format: PCR
Target Label: N/A
Internal Control: N/A

Full Validation Report

Analytical Sensitivity
Limit of detection: N/A

Species Detected1
Virus Name Species Name
Aconitum latent virus (AcLV)2 Carlavirus latensaconiti
American hop latent virus (AHLV)2 Carlavirus americanense
Atractylodes mottle virus (AtrMoV)2 Carlavirus atractylodis
Blueberry scorch virus (BlScV) Carlavirus vaccinii
Butterbur mosaic virus (ButMV)2 Carlavirus petasitis
Cactus virus 2 (CV-2)2 Carlavirus cacti
Caper latent virus (CapLV)2 Carlavirus latenscapparis
Carnation latent virus (CLV) Carlavirus latensdianthi
Chrysanthemum virus B (CVB) Carlavirus betachrysanthemi
Cole latent virus (CoLV)2 Carlavirus latensbrassicae
Coleus vein necrosis virus (CVNV) Carlavirus colei
Cowpea mild mottle virus (CPMMV) Carlavirus vignae
Cucumber vein-clearing virus (CuVCV)2 Carlavirus cucumis
Daphne virus S (DVS)2 Carlavirus sigmadaphnis
Gaillardia latent virus (GaLV)2 Carlavirus latensgaillardiae
Garlic common latent virus (GarCLV) Carlavirus latensallii
Helleborus mosaic virus (HeMV) Carlavirus hellebori
Helleborus net necrosis virus (HeNNV) Carlavirus necroretis
Hippeastrum latent virus (HLV)2 Carlavirus latenshippeastri
Hop latent virus (HpLV) Carlavirus latenshumuli
Hop mosaic virus (HpMV)2 Carlavirus humuli
Hydrangea chlorotic mottle virus (HdCMV) Carlavirus hydrangeae
Kalanchoe latent virus (KLV) Carlavirus latenskalanchoe
Ligustrum necrotic ringspot virus (LNRSV) Carlavirus necroligustri
Ligustrum virus A (LVA)2 Carlavirus alphaligustri
Lily symptomless virus (LSV) Carlavirus lilii
Melon yellowing-associated virus (MYaV)2 Carlavirus melonis
Mirabilis jalapa mottle virus (MjMV)2 Carlavirus mirabilis
Narcissus common latent virus (NCLV)2 Carlavirus latensnarcissi
Nerine latent virus (NeLV) Carlavirus latensnerinis
Passiflora latent virus (PLV)2 Carlavirus latenspassiflorae
Pea streak virus (PeSV) Carlavirus pisi
Phlox virus B (PhlVB)2 Carlavirus betaphlocis
Phlox virus M (PhlVM) Carlavirus miphlocis
Phlox virus S (PhlVS)2 Carlavirus sigmaphlocis
Poplar mosaic virus (PopMV)2 Carlavirus populi
Potato latent virus (PotLV) Carlavirus latensolani
Potato virus H (PVH)2 Carlavirus chisolani
Potato virus M (PVM) Carlavirus misolani
Potato virus P (PVP)2 Carlavirus pisolani
Potato virus S (PVS) Carlavirus sigmasolani
Red clover vein mosaic virus (RCVMV) Carlavirus trifolii
Sambucus virus C (SVC)2 Carlavirus gammasambuci
Sambucus virus D (SVD)2 Carlavirus deltasambuci
Sambucus virus E (SVE)2 Carlavirus epsilonsambuci
Shallot latent virus (SLV) Carlavirus latensascalonici
Sint-Jan onion latent virus (SJOLV)2 Carlavirus cornutum
Strawberry pseudo mild yellow edge virus (SPMYEV)2 Carlavirus fragariae
Sweet potato C6 virus (SC6V)2 Carlavirus ipomoeae
Sweet potato chlorotic fleck virus (SPCFV)2 Carlavirus chloroipomoeae
Verbena latent virus (VeLV)2 Carlavirus latensverbenae
Yam latent virus (YLV)2 Carlavirus latensdioscoreae

1The list above represents viruses that have been shown to be detected by this group PCR test. It also represents viruses that may be detected based on in silico analysis. If you have confirmed detection of a predicted virus detection or a virus not on this list, please contact us. We would like to work with you to further validate detection capabilities.
2Predicted detection by in silico analysis only

Cross-reactions
None known

For a list of validated hosts, please see the Full Validation Report.

  • Included Items
  • Technical Information
  • Documents