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ELISA Reagent Set for Broad bean wilt virus 1,2 (BBWV-1,2)

This ELISA test is a qualitative serological assay for the detection of Broad bean wilt virus 1 and 2 (BBWV-1,2) in ornamental leaves. BBWV-1,2 is a member of the Fabavirus genus known for their non-enveloped, icosahedral-shaped virus particles.

Click here for other product documents such as the User Guide or Buffer Formulations.

Included:

  • Capture antibody
  • Alkaline phosphatase enzyme conjugate
  • General extract buffer 3, GEB3
  • TWEEN® 20
  • 96-well microtiter plates, solid* (*strip in single plate set)
  • User Guide

Required but Not Included:

For a full list of included items, including sizes, please see the Content List.

TWEEN® is a registered trademark of Croda International PLC.

Validation Report Summary

Test Label: Alkaline Phosphatase
Test Format: Double Antibody Sandwich (DAS) ELISA
Capture Reagent: Polyclonal
Detection Reagent: Polyclonal

Full Validation Report


Analytical Sensitivity
Limit of detection: 1:51,200 dilution of infected tissue (pathogen titer unknown)

Isolate Names / Geographic Strain Locations
BBWV-1 PV-0221 (Italy) BBWV-1 PV-0548 (Syria)
BBWV-1 PV-0549 (Germany) BBWV-1 PV-0881 (Austria)
BBWV-1-Spinach (ATCC® PV-132™) (NY, USA) BBWV-2 PV-0537
BBWV-2 PV-0550 (Germany) BBWV-2 PV-0862 (Germany)
BBWV-2-Lettuce (ATCC® PV-131™) (NY, USA) BBWV-2-Yangzhou (China)1
BBWV-2-ZK2 (China)1 BBWV-2-ZK3 (China)1

1BBWV-2-Yangzhou, BBWV-2-ZK2, and BBWV-2-ZK3 have been externally reported to be detected.


Cross-reactions
None Known

For a list of validated hosts, please see the Full Validation Report.

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Reagent Set for Broad bean wilt virus 1 and 2 (BBWV 1,2), 96 testwells
SRA 46202/0096
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Reagent Set for Broad bean wilt virus 1 and 2 (BBWV 1,2), 500 testwells
SRA 46202/0500
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Reagent Set for Broad bean wilt virus 1 and 2 (BBWV 1,2), 1000 testwells
SRA 46202/1000
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Reagent Set for Broad bean wilt virus 1 and 2 (BBWV 1,2), 5000 testwells
SRA 46202/5000
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