PathoScreen® Kit for Tomato spotted wilt virus (TSWV)

This ELISA test is a qualitative serological assay for the detection of Tomato spotted wilt virus (TSWV) in tomato and pepper leaves as well as other ornamental and vegetable species. TSWV is a member of the Orthotospovirus genus known for their enveloped, spherical-shaped virus particles.

If you would prefer to add a field-deployable assay to your testing toolkit, please check out Agdia's TSWV ImmunoStrips®

Click here for other product documents such as the User Guide or Buffer Instructions.

Included:

  • Antibody-coated 96-well microtiter plates
  • Alkaline phosphatase enzyme conjugate
  • ECI buffer, 5X concentrate
  • PNP substrate tablets, 5 mg each
  • PNP substrate buffer, 5X concentrate
  • Positive control
  • PBST buffer, 20X concentrate, 50 mL
  • TWEEN® 20
  • General extract buffer, GEB
  • User Guide

Required but Not Included:

For a full list of included items, including sizes, please see the Content List.

TWEEN® is a registered trademark of Croda International PLC.

Validation Report Summary

Test Label: Alkaline Phosphatase
Test Format: Double Antibody Sandwich (DAS) ELISA
Capture Reagent: Monoclonal
Detection Reagent: Monoclonal

Full Validation Report

Analytical Sensitivity
Limit of detection: 1:24,300 dilution of infected tissue (pathogen titer unknown)

Isolate Names / Geographic Strain Locations
TSWV Brazil isolate TSWV Italy isolate
TSWV New Zealand isolate TSWV Spain isolate
TSWV USA isolate

Cross-reactions
Alstroemeria necrotic streak virus (ANSV)1 Groundnut ringspot virus (GRSV)
Tomato chlorotic spot virus (TCSV)

1ANSV has been externally reported to be detected in 2017 and 2019.

For a list of validated hosts, please see the Full Validation Report.

Option Unit Purchase
PathoScreen® Kit, Tomato spotted wilt virus (TSWV), 96 testwells
PSA 39300/0096
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PathoScreen® Kit, Tomato spotted wilt virus (TSWV), 288 testwells
PSA 39300/0288
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PathoScreen® Kit, Tomato spotted wilt virus (TSWV), 480 testwells
PSA 39300/0480
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