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PathoScreen® Kit for Tomato spotted wilt virus (TSWV)

This ELISA test is a qualitative serological assay for the detection of Tomato spotted wilt virus (TSWV) in tomato and pepper leaves as well as other ornamental and vegetable species. TSWV is a member of the Orthotospovirus genus known for their enveloped, spherical-shaped virus particles.

If you would prefer to add a field-deployable assay to your testing toolkit, please check out Agdia's TSWV ImmunoStrips®

Click here for other product documents such as the User Guide or Buffer Instructions.


  • Antibody-coated 96-well microtiter plates
  • Alkaline phosphatase enzyme conjugate
  • ECI buffer, 5X concentrate
  • PNP substrate tablets, 5 mg each
  • PNP substrate buffer, 5X concentrate
  • Positive control
  • PBST buffer, 20X concentrate, 50 mL
  • TWEEN® 20
  • General extract buffer, GEB
  • User Guide

Required but Not Included:

For a full list of included items, including sizes, please see the Content List.

TWEEN® is a registered trademark of Croda International PLC.

Validation Report Summary

Test Label: Alkaline Phosphatase
Test Format: Double Antibody Sandwich (DAS) ELISA
Capture Reagent: Monoclonal
Detection Reagent: Monoclonal

Full Validation Report

Analytical Sensitivity
Limit of detection: 1:24,300 dilution of infected tissue (pathogen titer unknown)

Isolate Names / Geographic Strain Locations
TSWV-CE (South Korea)1 TSWV-YN (China)1
TSWV Brazil isolate TSWV Italy isolate
TSWV New Zealand isolate TSWV Spain isolate
TSWV Sw-5 resistance breaking isolate1 TSWV USA isolate
1TSWV-CE, TSWV Sw-5 resistance breaking isolate, and TSWV-YN have been externally reported to be detected.

Alstroemeria necrotic streak virus (ANSV)1 Groundnut ringspot virus (GRSV)
Tomato chlorotic spot virus (TCSV)
1ANSV has been externally reported to be detected in 2017 and 2019.

For a list of validated hosts, please see the Full Validation Report.

Option Unit Purchase
PathoScreen® Kit, Tomato spotted wilt virus (TSWV), 96 testwells
PSA 39300/0096
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PathoScreen® Kit, Tomato spotted wilt virus (TSWV), 288 testwells
PSA 39300/0288
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PathoScreen® Kit, Tomato spotted wilt virus (TSWV), 480 testwells
PSA 39300/0480
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