PathoScreen® Kit for Tomato mosaic virus (ToMV)

This ELISA test is a qualitative serological assay for the detection of Tomato mosaic virus (ToMV) in ornamental and vegetable leaves. ToMV is a member of the Tobamovirus genus known for their rod-shaped virus particles.

Click here for other product documents such as the User Guide or Buffer Instructions.

Included:

  • Antibody-coated 96-well microtiter plates
  • Alkaline phosphatase enzyme conjugate
  • ECI buffer, 5X concentrate
  • PNP substrate tablets, 5 mg each
  • PNP substrate buffer, 5X concentrate
  • Synthetic Positive control
  • PBST buffer, 20X concentrate, 50 mL
  • TWEEN® 20
  • General extract buffer, GEB
  • User Guide

For a full list of included items, including sizes, please see the Content List.

TWEEN® is a registered trademark of Croda International PLC.

Validation Report Summary

Test Label: Alkaline Phosphatase
Test Format: Double Antibody Sandwich (DAS) ELISA
Capture Reagent: Polyclonal
Detection Reagent: Polyclonal

Full Validation Report


Analytical Sensitivity
Limit of detection: 1:109,350 dilution of infected tissue (pathogen titer unknown)

Isolate Names / Geographic Strain Locations
ToMV North Carolina strain ToMV PC-0135 (Germany)
ToMV PV-0135 (Germany) ToMV PV-0141 (France)
ToMV PV-0846 (Iran) ToMV PV-1180 (India)

Cross-reactions
Odontoglossum ringspot virus (ORSV) Pepper mild mottle virus (PMMoV)1
Ribgrass mosaic virus (RMV) Tobacco mild green mosaic virus (TMGMV)
Tobacco mosaic virus (TMV) Tomato brown rugose fruit virus (ToBRFV)

1Some isolates not detected.

For a list of validated hosts, please see the Full Validation Report.

Option Unit Purchase
PathoScreen® Kit, Tomato mosaic virus (ToMV), 96 testwells
PSA 35400/0096
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PathoScreen® Kit, Tomato mosaic virus (ToMV), 288 testwells
PSA 35400/0288
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PathoScreen® Kit, Tomato mosaic virus (ToMV), 480 testwells
PSA 35400/0480
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