ELISA Test Kits for ToMV (Tomato mosaic virus)
This ELISA test is a qualitative serological assay for the detection of Tomato mosaic virus (ToMV) in ornamental and vegetable leaves. ToMV is a member of the Tobamovirus genus known for their rod-shaped virus particles.
Click here for other product documents such as the Reagent Set User Guide or PathoScreen Kit User Guide, click on the Documents tab for the full list.
See below for a summary of our ELISA format offerings. Not all formats are available for every test. See the Included Items tab for full details.
- Reagent Set: Includes coating antibody, enzyme conjugate, and uncoated 96-well plate(s). Popular choice for established high-throughput laboratories.
- PathoScreen Complete Kit: Includes pre-coated 96-well plate(s), enzyme conjugate, positive control, and all required buffers. Easiest format to get started with ELISA and requires the least amount of time and labor to run.
- Capture Antibody: Only includes capture antibody. This is a partial component not usually ordered by itself. Unavailable on website. Call or email us to order.
- Enzyme Conjugate: Includes enzyme conjugate and 96-well plate(s). This is a partial component not usually ordered by itself. Unavailable on website. Call or email us to order.
Reagent Set
Included in Reagent Set:
- Capture antibody
- Alkaline phosphatase enzyme conjugate
- 96-well microtiter plates, solid* (*strip in single plate set)
- Reagent Set User Guide
Required but Not Included:
- Buffer Pack (ACC 00111)
- Carbonate coating buffer, 10X concentrate
- PNP substrate buffer, 5X concentrate
- PNP substrate tablets, 5 mg each
- PBST buffer, powder
- ECI buffer, 5X concentrate
- General extract buffer
- TWEEN® 20
- Buffer Formulations from Chemicals
- Logsheet
PathoScreen Kit
Included in PathoScreen Kit:
- Antibody-coated 96-well microtiter plates
- Alkaline phosphatase enzyme conjugate
- ECI buffer, 5X concentrate
- PNP substrate tablets, 5 mg each
- PNP substrate buffer, 5X concentrate
- Positive control
- PBST buffer, 20X concentrate, 50 mL
- TWEEN® 20
- General extract buffer, GEB
- PathoScreen User Guide
Required but Not Included:
Coating Antibody
Included in Coating Antibody:
- Capture antibody
- 96-well microtiter plates, solid
Required but Not Included:
- Only a partial component; see above for complete options
Enzyme Conjugate
Included in Enzyme Conjugate:
- Alkaline phosphatase enzyme conjugate
- Reagent Set User Guide
Required but Not Included:
- Only a partial component; see above for complete options
For a full list of included items, including sizes, please see the Content List.
TWEEN® is a registered trademark of Croda International PLC.
Validation Report Summary
|
Test Label: Alkaline Phosphatase |
Full Validation Report |
| Analytical Sensitivity |
|---|
| Limit of detection: 1:109,350 dilution of infected tissue (pathogen titer unknown) |
| Isolate Names / Geographic Strain Locations | |
|---|---|
| ToMV North Carolina strain | ToMV PC-0135 (Germany) |
| ToMV PV-0135 (Germany) | ToMV PV-0141 (France) |
| ToMV PV-0846 (Iran) | ToMV PV-1180 (India) |
| Cross-reactions | |
|---|---|
| Virus Name | Species Name |
| African eggplant-associated virus (AEaV)2 | N/A |
| Bell pepper mottle virus (BPeMV) | Tobamovirus maculacapsici |
| Chili pepper mild mottle virus (CPMMoV)3 | N/A |
| Odontoglossum ringspot virus (ORSV) | Tobamovirus odontoglossi |
| Paprika mild mottle virus (PaMMV) | Tobamovirus paprikae |
| Pepper mild mottle virus (PMMoV)1 | Tobamovirus capsici |
| Rehmannia mosaic virus (ReMV) | Tobamovirus rehmanniae |
| Ribgrass mosaic virus (RMV) | Tobamovirus plantagonis |
| Tobacco mild green mosaic virus (TMGMV) | Tobamovirus mititessellati |
| Tobacco mosaic virus (TMV) | Tobamovirus tabaci |
| Tomato brown rugose fruit virus (ToBRFV) | Tobamovirus fructirugosum |
| Tomato mottle mosaic virus (ToMMV) | Tobamovirus maculatessellati |
| Turnip vein-clearing virus (TVCV) | Tobamovirus rapae |
| Youcai mosaic virus (YoMV) | Tobamovirus youcai |
1Some isolates of Pepper mild mottle virus (PMMoV) not detected.
2African eggplant-associated virus (AEaV) has been reported to be a possible novel Tobamovirus.
3Chili pepper mild mottle virus (CPMMoV) has been reported to be a possible novel Tobamovirus.
For a list of validated hosts, please see the Full Validation Report.
| Option | Unit | Purchase |
|---|---|---|
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ToMV Reagent Set, 96 Testwells
SRA 35400/0096
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Shown in cart
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ToMV Reagent Set, 500 Testwells
SRA 35400/0500
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Shown in cart
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ToMV Reagent Set, 1000 Testwells
SRA 35400/1000
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Shown in cart
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ToMV Reagent Set, 5000 Testwells
SRA 35400/5000
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Shown in cart
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ToMV PathoScreen® Complete Kit, 96 Testwells
PSA 35400/0096
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Shown in cart
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ToMV PathoScreen® Complete Kit, 288 Testwells
PSA 35400/0288
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Shown in cart
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ToMV PathoScreen® Complete Kit, 480 Testwells
PSA 35400/0480
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Shown in cart
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