ELISA Reagent Set for Xylella fastidiosa (Xf)

Test Label: Peroxidase
Test Format: Double Antibody Sandwich (DAS) ELISA
Capture Reagent: Polyclonal
Detection Reagent: Polyclonal

Intended Use:

This ELISA test is a reliable tool for detection of Xylella fastidiosa, the causal agent of bacterial leaf scorch, Pierce's disease, and phony peach. This test system is based on a mixture of antibodies from three serologically distinct isolates of Xf. It detects all of a large panel of Xf isolates, including ones from almond, citrus, grape (8 isolates), elm, mulberry (2 isolates), oak (6 isolates), periwinkle, ragweed, and sycamore.

This system shows no cross-reaction with any healthy tissue tested. This ELISA test reacts positively with samples from oleander plants with the disease.

The highest populations of Xf bacteria occur in petioles of infected plants. When symptoms are evident on leaf surfaces, the bacteria responsible are most likely located in the petioles of affected leaves. We recommend that you use leaf petiole tissue for Xf testing when possible. All other parts of the plant can also be tested with this test system.

Limit of Detection: 2x106 cells/mL

Includes:

  • Capture antibody
  • Peroxidase enzyme conjugate
  • TMB peroxidase substrate solution, 1X
  • MRS component, 5X
  • 96-well microtiter plates, solid* (*strip in single plate set)
  • User Guide
Option Unit Purchase
Reagent Set for Xylella fastidiosa (Xf), 96 testwells
SRP 34501/0096
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Reagent Set for Xylella fastidiosa (Xf), 500 testwells
SRP 34501/0500
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Reagent Set for Xylella fastidiosa (Xf), 1000 testwells
SRP 34501/1000
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Reagent Set for Xylella fastidiosa (Xf), 5000 testwells
SRP 34501/5000
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