ELISA Reagent Set for Clavibacter michiganensis subsp. michiganensis (Cmm)

This ELISA test is a qualitative serological assay for the detection of Clavibacter michiganensis subsp. michiganensis (Cmm), the causal agent of bacterial canker, in tomato leaves. Cmm is a member of the Clavibacter genus known for their aerobic, non-motile, Gram-positive, non-sporing, curved rod shaped bacteria.

If you would prefer to add a field-deployable assay to your testing toolkit, please check out Agdia's Cmm ImmunoStrips®

Click here for other product documents such as the User Guide or Buffer Formulations.

Included:

  • Capture antibody
  • Alkaline phosphatase enzyme conjugate
  • 96-well microtiter plates, solid* (*strip in single plate set)
  • User Guide

Required but Not Included:

For a full list of included items, including sizes, please see the Content List.

TWEEN® is a registered trademark of Croda International PLC.

Validation Report Summary

Test Label: Alkaline Phosphatase
Test Format: Double Antibody Sandwich (DAS) ELISA
Capture Reagent: Polyclonal
Detection Reagent: Polyclonal

Full Validation Report


Analytical Sensitivity
Limit of detection: 4.0x104 CFU/mL

Isolate Names / Geographic Strain Locations
This assay was designed to detect all strains and isolates of Cmm. Thirty-one distinct samples of Cmm have been experimentally proven to be detected including samples of isolate 557B0-1.

Cross-reactions
Clavibacter michiganensis subsp. insidiosus (Cmi)1 Clavibacter michiganensis subsp. nebraskensis (Cmn)
Clavibacter michiganensis subsp. sepedonicus (Cms) (mucoid) Clavibacter michiganensis pv. tessellarius (Cmt)

1Cross-reaction observed in 2 out of 3 samples.

For a list of validated hosts, please see the Full Validation Report.

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Reagent Set for Clavibacter michiganensis subsp. michiganensis (Cmm), 96 testwells
SRA 44000/0096
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Reagent Set for Clavibacter michiganensis subsp. michiganensis (Cmm), 500 testwells
SRA 44000/0500
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Reagent Set for Clavibacter michiganensis subsp. michiganensis (Cmm), 1000 testwells
SRA 44000/1000
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Reagent Set for Clavibacter michiganensis subsp. michiganensis (Cmm), 5000 testwells
SRA 44000/5000
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