ELISA Reagent Set for Blueberry scorch virus (BlScV)

This ELISA test is a qualitative serological assay for the detection of Blueberry scorch virus (BlScV) in blueberry and cranberry flowers and leaves. BlScV is a member of the Carlavirus genus known for their flexuous rod-shaped virus particles.

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Included:

  • Capture antibody
  • Alkaline phosphatase enzyme conjugate
  • RUB3 Enzyme conjugate diluent, 1X Ready to use
  • General extract buffer 3, GEB3
  • TWEEN® 20
  • 96-well microtiter plates, solid* (*strip in single plate set)
  • User Guide

Required but Not Included:

For a full list of included items, including sizes, please see the Content List.

TWEEN® is a registered trademark of Croda International PLC.

Validation Report Summary

Test Label: Alkaline Phosphatase
Test Format: Double Antibody Sandwich (DAS) ELISA
Capture Reagent: Polyclonal
Detection Reagent: Polyclonal

Full Validation Report


Analytical Sensitivity
Limit of detection: 1:320 dilution of infected tissue (pathogen titer unknown)

Isolate Names / Geographic Strain Locations
This assay was designed to detect all strains and isolates of BlScV. Thirty-five distinct samples of BlScV have been experimentally proven to be detected including samples of the Northwest strain and the New Jersey strain (sheep pen hill disease).

Cross-reactions
Chrysanthemum virus B (CVB) Potato virus M (PVM)
Potato virus S (PVS)

For a list of validated hosts, please see the Full Validation Report.